Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

1E3E

Mouse class II alcohol dehydrogenase complex with NADH

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0001523	biological_process	retinoid metabolic process
A	0003960	molecular_function	NADPH:quinone reductase activity
A	0004022	molecular_function	alcohol dehydrogenase (NAD+) activity
A	0004024	molecular_function	alcohol dehydrogenase (NAD+) activity, zinc-dependent
A	0004032	molecular_function	aldose reductase (NADPH) activity
A	0004745	molecular_function	all-trans-retinol dehydrogenase (NAD+) activity
A	0005503	molecular_function	all-trans retinal binding
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006066	biological_process	alcohol metabolic process
A	0006067	biological_process	ethanol metabolic process
A	0006069	biological_process	obsolete ethanol oxidation
A	0006081	biological_process	cellular aldehyde metabolic process
A	0006629	biological_process	lipid metabolic process
A	0008270	molecular_function	zinc ion binding
A	0010430	biological_process	fatty acid omega-oxidation
A	0016491	molecular_function	oxidoreductase activity
A	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
A	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
A	0019115	molecular_function	benzaldehyde dehydrogenase [NAD(P)+] activity
A	0019841	molecular_function	retinol binding
A	0035276	molecular_function	ethanol binding
A	0042572	biological_process	retinol metabolic process
A	0044282	biological_process	small molecule catabolic process
A	0046164	biological_process	alcohol catabolic process
A	0046294	biological_process	formaldehyde catabolic process
A	0046872	molecular_function	metal ion binding
A	0051287	molecular_function	NAD binding
A	0051903	molecular_function	S-(hydroxymethyl)glutathione dehydrogenase [NAD(P)+] activity
A	1901661	biological_process	quinone metabolic process
B	0001523	biological_process	retinoid metabolic process
B	0003960	molecular_function	NADPH:quinone reductase activity
B	0004022	molecular_function	alcohol dehydrogenase (NAD+) activity
B	0004024	molecular_function	alcohol dehydrogenase (NAD+) activity, zinc-dependent
B	0004032	molecular_function	aldose reductase (NADPH) activity
B	0004745	molecular_function	all-trans-retinol dehydrogenase (NAD+) activity
B	0005503	molecular_function	all-trans retinal binding
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006066	biological_process	alcohol metabolic process
B	0006067	biological_process	ethanol metabolic process
B	0006069	biological_process	obsolete ethanol oxidation
B	0006081	biological_process	cellular aldehyde metabolic process
B	0006629	biological_process	lipid metabolic process
B	0008270	molecular_function	zinc ion binding
B	0010430	biological_process	fatty acid omega-oxidation
B	0016491	molecular_function	oxidoreductase activity
B	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
B	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
B	0019115	molecular_function	benzaldehyde dehydrogenase [NAD(P)+] activity
B	0019841	molecular_function	retinol binding
B	0035276	molecular_function	ethanol binding
B	0042572	biological_process	retinol metabolic process
B	0044282	biological_process	small molecule catabolic process
B	0046164	biological_process	alcohol catabolic process
B	0046294	biological_process	formaldehyde catabolic process
B	0046872	molecular_function	metal ion binding
B	0051287	molecular_function	NAD binding
B	0051903	molecular_function	S-(hydroxymethyl)glutathione dehydrogenase [NAD(P)+] activity
B	1901661	biological_process	quinone metabolic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN A 378

Chain	Residue
A	CYS46
A	HIS67
A	CYS178
A	HOH2222

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN A 379

Chain	Residue
A	CYS97
A	CYS100
A	CYS103
A	CYS111

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN B 378

Chain	Residue
B	HIS67
B	CYS178
B	HOH2280
B	CYS46

site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE ZN B 379

Chain	Residue
B	CYS97
B	LYS98
B	CYS100
B	CYS103
B	CYS111

site_id	AC5
Number of Residues	23
Details	BINDING SITE FOR RESIDUE NAI A 377

Chain	Residue
A	THR48
A	SER182
A	GLY203
A	GLY205
A	CYS206
A	VAL207
A	ASP227
A	CYS272
A	THR278
A	VAL296
A	GLY297
A	THR319
A	PHE320
A	PHE321
A	ARG371
A	HOH2151
A	HOH2171
A	HOH2217
A	HOH2218
A	HOH2219
A	HOH2220
A	HOH2221
B	ASP362

site_id	AC6
Number of Residues	27
Details	BINDING SITE FOR RESIDUE NAI B 377

Chain	Residue
B	PRO47
B	THR48
B	SER182
B	GLY203
B	GLY205
B	CYS206
B	VAL207
B	ASP227
B	ILE228
B	LYS232
B	CYS272
B	THR278
B	VAL296
B	GLY297
B	THR319
B	PHE320
B	PHE321
B	ARG371
B	HOH2163
B	HOH2267
B	HOH2273
B	HOH2274
B	HOH2275
B	HOH2276
B	HOH2277
B	HOH2278
B	HOH2279

Functional Information from PROSITE/UniProt

site_id	PS00059
Number of Residues	15
Details	ADH_ZINC Zinc-containing alcohol dehydrogenases signature. GHEcAGIvesvGpgV

Chain	Residue	Details
A	GLY66-VAL80

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	14
Details	BINDING:

Chain	Residue	Details
A	CYS46
B	CYS97
B	CYS100
B	CYS103
B	CYS111
B	CYS178
A	HIS67
A	CYS97
A	CYS100
A	CYS103
A	CYS111
A	CYS178
B	CYS46
B	HIS67

site_id	SWS_FT_FI2
Number of Residues	14
Details	BINDING: BINDING => ECO:0000269\|PubMed:10970744

Chain	Residue	Details
A	THR48
B	ASP227
B	LYS232
B	VAL296
B	THR319
B	ARG371
A	GLY203
A	ASP227
A	LYS232
A	VAL296
A	THR319
A	ARG371
B	THR48
B	GLY203

Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1teh

Chain	Residue	Details
A	THR48
A	PRO47

site_id	CSA2
Number of Residues	2
Details	Annotated By Reference To The Literature 1teh

Chain	Residue	Details
B	THR48
B	PRO47

site_id	MCSA1
Number of Residues	6
Details	M-CSA 500

Chain	Residue	Details
A	CYS46	metal ligand
A	PRO47	steric role
A	THR48	proton shuttle (general acid/base)
A	HIS67	metal ligand
A	CYS178	metal ligand
A	SER182	proton shuttle (general acid/base)

site_id	MCSA2
Number of Residues	6
Details	M-CSA 500

Chain	Residue	Details
B	CYS46	metal ligand
B	PRO47	steric role
B	THR48	proton shuttle (general acid/base)
B	HIS67	metal ligand
B	CYS178	metal ligand
B	SER182	proton shuttle (general acid/base)

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)