1E22

LYSYL-TRNA SYNTHETASE (LYSU) HEXAGONAL FORM complexed with lysine and the non-hydrolysable atp analogue amp-pcp

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000049	molecular_function	tRNA binding
A	0000166	molecular_function	nucleotide binding
A	0000287	molecular_function	magnesium ion binding
A	0003676	molecular_function	nucleic acid binding
A	0004812	molecular_function	aminoacyl-tRNA ligase activity
A	0004824	molecular_function	lysine-tRNA ligase activity
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006412	biological_process	translation
A	0006418	biological_process	tRNA aminoacylation for protein translation
A	0006430	biological_process	lysyl-tRNA aminoacylation
A	0016020	cellular_component	membrane
A	0016874	molecular_function	ligase activity
A	0034605	biological_process	cellular response to heat
A	0036260	biological_process	RNA capping
A	0042803	molecular_function	protein homodimerization activity
A	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG A 515

Chain	Residue
A	ARG412
A	GLU414
A	GLU421
A	ACP512
A	MG517

---

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG A 516

Chain	Residue
A	GLU264
A	ACP512
A	HOH2172
A	HOH2303

---

site_id	AC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG A 517

Chain	Residue
A	GLU421
A	ACP512
A	MG515

---

site_id	AC4
Number of Residues	13
Details	BINDING SITE FOR RESIDUE LYS A 510

Chain	Residue
A	GLY216
A	ALA217
A	GLU240
A	ARG262
A	MET276
A	GLU278
A	TYR280
A	ASN424
A	PHE426
A	GLU428
A	GLY473
A	ACP512
A	GOL523

---

site_id	AC5
Number of Residues	21
Details	BINDING SITE FOR RESIDUE ACP A 512

Chain	Residue
A	ARG262
A	GLU264
A	ARG269
A	HIS270
A	ASN271
A	PHE274
A	GLU380
A	GLU414
A	GLU421
A	ILE422
A	GLY477
A	ARG480
A	ILE491
A	LYS510
A	MG515
A	MG516
A	MG517
A	GOL523
A	HOH2258
A	HOH2302
A	HOH2303

---

site_id	AC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 520

Chain	Residue
A	TYR312
A	HIS315
A	ILE384
A	GLY418
A	ARG420
A	HIS489

---

site_id	AC7
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL A 521

Chain	Residue
A	ARG194
A	ARG194
A	GLU204
A	GLU206
A	ILE257
A	ASN258
A	ASN258
A	HOH2169

---

site_id	AC8
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 522

Chain	Residue
A	TYR286
A	GLU391
A	ASN403
A	THR410
A	ASP411
A	ARG412

---

site_id	AC9
Number of Residues	10
Details	BINDING SITE FOR RESIDUE GOL A 523

Chain	Residue
A	ALA217
A	SER218
A	VAL396
A	ARG412
A	ASN424
A	PHE426
A	LYS510
A	ACP512
A	HOH2152
A	HOH2305

---

site_id	BC1
Number of Residues	12
Details	BINDING SITE FOR RESIDUE GOL A 524

Chain	Residue
A	PRO214
A	GLY215
A	ALA217
A	SER218
A	ALA219
A	PRO221
A	ILE237
A	LYS446
A	GLU452
A	ALA453
A	MET454
A	PHE455

---

site_id	BC2
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL A 525

Chain	Residue
A	VAL33
A	LYS333
A	ARG402
A	ASN403
A	ASP404
A	HOH2250
A	HOH2306

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	Binding site: {}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"18723842","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	3
Details	Annotated By Reference To The Literature 1asy

Chain	Residue	Details
A	ARG480
A	GLU278
A	ARG262

---

site_id	CSA2
Number of Residues	1
Details	Annotated By Reference To The Literature 1asy

Chain	Residue	Details
A	ARG262

---