1DWI

Study on radiation damage on a cryocooled crystal. Part 5: Structure after irradiation with 54.0*10e15 photons/mm2

Functional Information from GO Data

Chain	GOid	namespace	contents
M	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
M	0005773	cellular_component	vacuole
M	0005975	biological_process	carbohydrate metabolic process
M	0008422	molecular_function	beta-glucosidase activity
M	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
M	0019137	molecular_function	thioglucosidase activity
M	0046872	molecular_function	metal ion binding

Functional Information from PROSITE/UniProt

site_id	PS00572
Number of Residues	9
Details	GLYCOSYL_HYDROL_F1_1 Glycosyl hydrolases family 1 active site. IYVTENGIS

Chain	Residue	Details
M	ILE405-SER413

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site_id	PS00653
Number of Residues	15
Details	GLYCOSYL_HYDROL_F1_2 Glycosyl hydrolases family 1 N-terminal signature. FiFGvAsSAYQiEgT

Chain	Residue	Details
M	PHE29-THR43

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Nucleophile

Chain	Residue	Details
M	GLU409

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site_id	SWS_FT_FI2
Number of Residues	5
Details	BINDING:

Chain	Residue	Details
M	GLN39
M	HIS56
M	ASP70
M	HIS141
M	ASN186

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site_id	SWS_FT_FI3
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269|PubMed:10978344

Chain	Residue	Details
M	GLN187
M	ARG259

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site_id	SWS_FT_FI4
Number of Residues	3
Details	BINDING: BINDING => ECO:0000250

Chain	Residue	Details
M	TYR330
M	TRP457
M	GLU464

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site_id	SWS_FT_FI5
Number of Residues	11
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:10978344, ECO:0000269|PubMed:15889170, ECO:0000269|PubMed:9195886

Chain	Residue	Details
M	ASN21
M	ASN361
M	ASN482
M	ASN60
M	ASN90
M	ASN218
M	ASN244
M	ASN265
M	ASN292
M	ASN343
M	ASN346

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Catalytic Information from CSA

site_id	CSA1
Number of Residues	3
Details	Annotated By Reference To The Literature 1cbg

Chain	Residue	Details
M	GLN187
M	ASN328
M	GLU409

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site_id	CSA2
Number of Residues	2
Details	Annotated By Reference To The Literature 1cbg

Chain	Residue	Details
M	GLN187
M	GLU409

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site_id	CSA3
Number of Residues	6
Details	Annotated By Reference To The Literature 1cbg

Chain	Residue	Details
M	GLN187
M	ASN328
M	ARG95
M	GLU409
M	TYR330
M	SER190

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site_id	MCSA1
Number of Residues	6
Details	M-CSA 460

Chain	Residue	Details
M	ARG95	electrostatic stabiliser
M	GLN187	electrostatic stabiliser, modifies pKa, steric role
M	SER190	electrostatic stabiliser, steric role
M	ASN328	electrostatic stabiliser, steric role
M	TYR330	transition state stabiliser
M	GLU409	covalently attached, electrostatic stabiliser

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