1DL2

CRYSTAL STRUCTURE OF CLASS I ALPHA-1,2-MANNOSIDASE FROM SACCHAROMYCES CEREVISIAE AT 1.54 ANGSTROM RESOLUTION

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004559	molecular_function	alpha-mannosidase activity
A	0004571	molecular_function	mannosyl-oligosaccharide 1,2-alpha-mannosidase activity
A	0005509	molecular_function	calcium ion binding
A	0005768	cellular_component	endosome
A	0005783	cellular_component	endoplasmic reticulum
A	0005789	cellular_component	endoplasmic reticulum membrane
A	0005802	cellular_component	trans-Golgi network
A	0005975	biological_process	carbohydrate metabolic process
A	0006486	biological_process	protein glycosylation
A	0008152	biological_process	metabolic process
A	0016020	cellular_component	membrane
A	0016787	molecular_function	hydrolase activity
A	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
A	0035977	biological_process	protein deglycosylation involved in glycoprotein catabolic process
A	0036503	biological_process	ERAD pathway
A	0046872	molecular_function	metal ion binding

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Proton donor => ECO:0000250|UniProtKB:P31723

Chain	Residue	Details
A	GLU399

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site_id	SWS_FT_FI2
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269|PubMed:10675327

Chain	Residue	Details
A	THR525

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site_id	SWS_FT_FI3
Number of Residues	3
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|Ref.7, ECO:0007744|PDB:1G6I

Chain	Residue	Details
A	ASN96
A	ASN155
A	ASN224

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	4
Details	M-CSA 19

Chain	Residue	Details
A	GLU132	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
A	ARG136	activator, electrostatic stabiliser, hydrogen bond donor
A	ASP275	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
A	GLU435	activator

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