1DJ2

STRUCTURES OF ADENYLOSUCCINATE SYNTHETASE FROM TRITICUM AESTIVUM AND ARABIDOPSIS THALIANA

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0000287	molecular_function	magnesium ion binding
A	0004019	molecular_function	adenylosuccinate synthase activity
A	0005525	molecular_function	GTP binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006164	biological_process	purine nucleotide biosynthetic process
A	0006167	biological_process	AMP biosynthetic process
A	0009507	cellular_component	chloroplast
A	0009536	cellular_component	plastid
A	0009570	cellular_component	chloroplast stroma
A	0016874	molecular_function	ligase activity
A	0044208	biological_process	'de novo' AMP biosynthetic process
A	0046872	molecular_function	metal ion binding
A	0048046	cellular_component	apoplast
B	0000166	molecular_function	nucleotide binding
B	0000287	molecular_function	magnesium ion binding
B	0004019	molecular_function	adenylosuccinate synthase activity
B	0005525	molecular_function	GTP binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006164	biological_process	purine nucleotide biosynthetic process
B	0006167	biological_process	AMP biosynthetic process
B	0009507	cellular_component	chloroplast
B	0009536	cellular_component	plastid
B	0009570	cellular_component	chloroplast stroma
B	0016874	molecular_function	ligase activity
B	0044208	biological_process	'de novo' AMP biosynthetic process
B	0046872	molecular_function	metal ion binding
B	0048046	cellular_component	apoplast

Functional Information from PDB Data

site_id	AC1
Number of Residues	15
Details	BINDING SITE FOR RESIDUE GDP A 500

Chain	Residue
A	GLY30
A	TYR62
A	LYS349
A	ASP351
A	GLY432
A	ILE433
A	GLY434
A	ASP31
A	GLY33
A	LYS34
A	GLY35
A	LYS36
A	GLY58
A	HIS59
A	THR60

---

site_id	AC2
Number of Residues	16
Details	BINDING SITE FOR RESIDUE GDP B 600

Chain	Residue
B	GLY30
B	ASP31
B	GLY33
B	LYS34
B	GLY35
B	LYS36
B	GLY58
B	HIS59
B	THR60
B	TYR62
B	LYS349
B	ASP351
B	GLY432
B	ILE433
B	GLY434
B	PRO435

---

Functional Information from PROSITE/UniProt

site_id	PS00513
Number of Residues	12
Details	ADENYLOSUCCIN_SYN_2 Adenylosuccinate synthetase active site. GIGPaYssKviR

Chain	Residue	Details
A	GLY151-ARG162

---

site_id	PS01266
Number of Residues	8
Details	ADENYLOSUCCIN_SYN_1 Adenylosuccinate synthetase GTP-binding site. QWGDEGKG

Chain	Residue	Details
A	GLN28-GLY35

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: Proton acceptor => ECO:0000255|HAMAP-Rule:MF_03125

Chain	Residue	Details
A	ASP31
B	ASP31

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	ACT_SITE: Proton donor => ECO:0000255|HAMAP-Rule:MF_03125

Chain	Residue	Details
A	HIS59
B	HIS59

---

site_id	SWS_FT_FI3
Number of Residues	6
Details	BINDING:

Chain	Residue	Details
A	GLY30
A	LYS349
A	GLY432
B	GLY30
B	LYS349
B	GLY432

---

site_id	SWS_FT_FI4
Number of Residues	10
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_03125

Chain	Residue	Details
A	ASP31
B	ARG323
A	GLY58
A	ARG162
A	THR317
A	ARG323
B	ASP31
B	GLY58
B	ARG162
B	THR317

---

site_id	SWS_FT_FI5
Number of Residues	10
Details	BINDING: in other chain => ECO:0000255|HAMAP-Rule:MF_03125

Chain	Residue	Details
A	ASN56
B	ARG321
A	THR148
A	GLN242
A	THR257
A	ARG321
B	ASN56
B	THR148
B	GLN242
B	THR257

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	3
Details	Annotated By Reference To The Literature 1gim

Chain	Residue	Details
A	GLN242
A	ASP31
A	HIS59

---

site_id	CSA2
Number of Residues	3
Details	Annotated By Reference To The Literature 1gim

Chain	Residue	Details
B	GLN242
B	ASP31
B	HIS59

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