1DB3

E.COLI GDP-MANNOSE 4,6-DEHYDRATASE

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0008446	molecular_function	GDP-mannose 4,6-dehydratase activity
A	0009242	biological_process	colanic acid biosynthetic process
A	0016829	molecular_function	lyase activity
A	0019673	biological_process	GDP-mannose metabolic process
A	0042351	biological_process	'de novo' GDP-L-fucose biosynthetic process
A	0042803	molecular_function	protein homodimerization activity
A	0070401	molecular_function	NADP+ binding

Functional Information from PROSITE/UniProt

site_id	PS00061
Number of Residues	29
Details	ADH_SHORT Short-chain dehydrogenases/reductases family signature. PqkettpfypRspYAVAKLYAyWITvNYR

Chain	Residue	Details
A	PRO143-ARG171

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	Active site: {"evidences":[{"source":"PubMed","id":"10673432","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI2
Number of Residues	2
Details	Active site: {"description":"Nucleophile","evidences":[{"source":"PubMed","id":"10673432","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI3
Number of Residues	13
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00955","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI4
Number of Residues	1
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

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Catalytic Information from CSA

site_id	CSA1
Number of Residues	4
Details	a catalytic site defined by CSA, PubMed 20139699, 19459932, 17974560

Chain	Residue	Details
A	GLU134
A	LYS160
A	THR132
A	TYR156

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site_id	MCSA1
Number of Residues	4
Details	M-CSA 487

Chain	Residue	Details
A	THR132	proton shuttle (general acid/base)
A	GLU134	proton shuttle (general acid/base)
A	TYR156	electrostatic stabiliser, proton shuttle (general acid/base)
A	LYS160	electrostatic stabiliser, modifies pKa

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