1CJK

COMPLEX OF GS-ALPHA WITH THE CATALYTIC DOMAINS OF MAMMALIAN ADENYLYL CYCLASE: COMPLEX WITH ADENOSINE 5'-(ALPHA THIO)-TRIPHOSPHATE (RP), MG, AND MN

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0009190	biological_process	cyclic nucleotide biosynthetic process
A	0016849	molecular_function	phosphorus-oxygen lyase activity
A	0035556	biological_process	intracellular signal transduction
B	0009190	biological_process	cyclic nucleotide biosynthetic process
B	0016849	molecular_function	phosphorus-oxygen lyase activity
B	0035556	biological_process	intracellular signal transduction
C	0003924	molecular_function	GTPase activity
C	0005525	molecular_function	GTP binding
C	0007165	biological_process	signal transduction
C	0007186	biological_process	G protein-coupled receptor signaling pathway
C	0019001	molecular_function	guanyl nucleotide binding
C	0031683	molecular_function	G-protein beta/gamma-subunit complex binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG A 581

Chain	Residue
A	HOH53
A	ASP396
A	ASP440
A	MN582
A	TAT801

---

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MN A 582

Chain	Residue
A	TAT801
A	ASP396
A	ILE397
A	ASP440
A	MG581

---

site_id	AC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG C 403

Chain	Residue
C	SER54
C	THR204
C	GSP405
C	HOH409
C	HOH412

---

site_id	AC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CL C 404

Chain	Residue
C	SER51
C	ALA249
C	HOH406

---

site_id	AC5
Number of Residues	23
Details	BINDING SITE FOR RESIDUE GSP C 405

Chain	Residue
C	GLU50
C	SER51
C	GLY52
C	LYS53
C	SER54
C	THR55
C	ASP173
C	LEU198
C	ARG199
C	ARG201
C	LEU203
C	THR204
C	GLY226
C	ASN292
C	LYS293
C	ASP295
C	LEU296
C	CYS365
C	ALA366
C	VAL367
C	MG403
C	HOH409
C	HOH412

---

site_id	AC6
Number of Residues	8
Details	BINDING SITE FOR RESIDUE FOK A 701

Chain	Residue
A	VAL506
A	SER508
A	THR512
B	PHE895
B	TYR899
B	ILE940
B	GLY941
B	SER942

---

site_id	AC7
Number of Residues	22
Details	BINDING SITE FOR RESIDUE TAT A 801

Chain	Residue
A	HOH11
A	HOH53
A	ASP396
A	ILE397
A	GLU398
A	GLY399
A	PHE400
A	THR401
A	GLY439
A	ASP440
A	ARG484
A	MG581
A	MN582
B	LYS938
B	ILE940
B	ASP1018
B	ILE1019
B	VAL1024
B	ASN1025
B	SER1028
B	ARG1029
B	LYS1065

---

site_id	AC8
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MES A 901

Chain	Residue
A	TYR553
A	GLU556
B	LYS1067
B	GLY1068
B	ASP1069
B	LEU1070

---

Functional Information from PROSITE/UniProt

site_id	PS00452
Number of Residues	24
Details	GUANYLATE_CYCLASE_1 Guanylate cyclase signature. GVL.GlrkwqFdVWSNDVTlanhmE

Chain	Residue	Details
A	GLY495-GLU518
B	GLY1008-ASP1031

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	127
Details	Domain: {"description":"Guanylate cyclase 1","evidences":[{"source":"PROSITE-ProRule","id":"PRU00099","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"PubMed","id":"10427002","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"11087399","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"16766715","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"19243146","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	3
Details	Binding site: {"evidences":[{"source":"PubMed","id":"10427002","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	20
Details	Region: {"description":"Interaction with GNAS","evidences":[{"source":"PubMed","id":"11087399","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"10427002","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"11087399","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"15591060","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"16766715","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"19243146","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"10427002","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1CJK","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3MAA","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	13
Details	Region: {"description":"G1 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	8
Details	Region: {"description":"G2 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	9
Details	Region: {"description":"G3 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	7
Details	Region: {"description":"G4 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	5
Details	Region: {"description":"G5 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	23
Details	Binding site: {"evidences":[{"source":"PubMed","id":"10427002","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"11087399","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"15591060","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"16766715","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"19243146","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"9395396","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"9417641","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"10427002","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"11087399","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"15591060","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19243146","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9395396","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9417641","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"16766715","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P63092","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI15
Number of Residues	2
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"UniProtKB","id":"P63092","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	1
Details	Annotated By Reference To The Literature 1ab8

Chain	Residue	Details
B	ARG1029

---

site_id	CSA2
Number of Residues	1
Details	Annotated By Reference To The Literature 1ab8

Chain	Residue	Details
A	HIS516

---

site_id	CSA3
Number of Residues	4
Details	Annotated By Reference To The Literature 1ab8

Chain	Residue	Details
C	THR204
C	ARG201
C	GLU50
C	GLN227

---

site_id	CSA4
Number of Residues	1
Details	Annotated By Reference To The Literature 1ab8

Chain	Residue	Details
C	GLN227

---

site_id	MCSA1
Number of Residues	2
Details	M-CSA 58

Chain	Residue	Details
B	ARG1029	electrostatic stabiliser
B	LYS1065	electrostatic stabiliser
A	ASP440	metal ligand

---