1CJ0
CRYSTAL STRUCTURE OF RABBIT CYTOSOLIC SERINE HYDROXYMETHYLTRANSFERASE AT 2.8 ANGSTROM RESOLUTION
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000900 | molecular_function | mRNA regulatory element binding translation repressor activity |
| A | 0004372 | molecular_function | glycine hydroxymethyltransferase activity |
| A | 0005634 | cellular_component | nucleus |
| A | 0005654 | cellular_component | nucleoplasm |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005739 | cellular_component | mitochondrion |
| A | 0005829 | cellular_component | cytosol |
| A | 0006544 | biological_process | glycine metabolic process |
| A | 0006563 | biological_process | L-serine metabolic process |
| A | 0006565 | biological_process | L-serine catabolic process |
| A | 0006730 | biological_process | one-carbon metabolic process |
| A | 0009113 | biological_process | purine nucleobase biosynthetic process |
| A | 0016740 | molecular_function | transferase activity |
| A | 0017148 | biological_process | negative regulation of translation |
| A | 0019264 | biological_process | glycine biosynthetic process from serine |
| A | 0030170 | molecular_function | pyridoxal phosphate binding |
| A | 0035999 | biological_process | tetrahydrofolate interconversion |
| A | 0036094 | molecular_function | small molecule binding |
| A | 0042802 | molecular_function | identical protein binding |
| A | 0042803 | molecular_function | protein homodimerization activity |
| A | 0046653 | biological_process | tetrahydrofolate metabolic process |
| A | 0046655 | biological_process | folic acid metabolic process |
| A | 0048027 | molecular_function | mRNA 5'-UTR binding |
| A | 0051289 | biological_process | protein homotetramerization |
| A | 0070905 | molecular_function | serine binding |
| A | 1904482 | biological_process | cellular response to tetrahydrofolate |
| B | 0000900 | molecular_function | mRNA regulatory element binding translation repressor activity |
| B | 0004372 | molecular_function | glycine hydroxymethyltransferase activity |
| B | 0005634 | cellular_component | nucleus |
| B | 0005654 | cellular_component | nucleoplasm |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005739 | cellular_component | mitochondrion |
| B | 0005829 | cellular_component | cytosol |
| B | 0006544 | biological_process | glycine metabolic process |
| B | 0006563 | biological_process | L-serine metabolic process |
| B | 0006565 | biological_process | L-serine catabolic process |
| B | 0006730 | biological_process | one-carbon metabolic process |
| B | 0009113 | biological_process | purine nucleobase biosynthetic process |
| B | 0016740 | molecular_function | transferase activity |
| B | 0017148 | biological_process | negative regulation of translation |
| B | 0019264 | biological_process | glycine biosynthetic process from serine |
| B | 0030170 | molecular_function | pyridoxal phosphate binding |
| B | 0035999 | biological_process | tetrahydrofolate interconversion |
| B | 0036094 | molecular_function | small molecule binding |
| B | 0042802 | molecular_function | identical protein binding |
| B | 0042803 | molecular_function | protein homodimerization activity |
| B | 0046653 | biological_process | tetrahydrofolate metabolic process |
| B | 0046655 | biological_process | folic acid metabolic process |
| B | 0048027 | molecular_function | mRNA 5'-UTR binding |
| B | 0051289 | biological_process | protein homotetramerization |
| B | 0070905 | molecular_function | serine binding |
| B | 1904482 | biological_process | cellular response to tetrahydrofolate |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 14 |
| Details | BINDING SITE FOR RESIDUE PLP A 2291 |
| Chain | Residue |
| A | SER97 |
| A | GLY98 |
| A | SER99 |
| A | HIS126 |
| A | SER175 |
| A | ASP200 |
| A | ALA202 |
| A | HIS203 |
| A | THR226 |
| A | HIS228 |
| A | LYS229 |
| B | TYR55 |
| B | GLY262 |
| B | GLY263 |
| site_id | AC2 |
| Number of Residues | 15 |
| Details | BINDING SITE FOR RESIDUE PLP B 2291 |
| Chain | Residue |
| A | TYR55 |
| A | GLY262 |
| A | GLY263 |
| B | SER97 |
| B | GLY98 |
| B | SER99 |
| B | HIS126 |
| B | THR174 |
| B | SER175 |
| B | ASP200 |
| B | ALA202 |
| B | HIS203 |
| B | THR226 |
| B | HIS228 |
| B | LYS229 |
| site_id | CTA |
| Number of Residues | 6 |
| Details | RESIDUES COMPRISING ACTIVE SITE FOR MOLECULE A |
| Chain | Residue |
| B | GLU57 |
| B | TYR65 |
| B | ARG363 |
| A | HIS126 |
| A | THR226 |
| A | HIS228 |
| site_id | CTB |
| Number of Residues | 6 |
| Details | RESIDUES COMPRISING ACTIVE SITE FOR MOLECULE B |
| Chain | Residue |
| B | HIS126 |
| B | THR226 |
| B | HIS228 |
| A | GLU57 |
| A | TYR65 |
| A | ARG363 |
Functional Information from PROSITE/UniProt
| site_id | PS00096 |
| Number of Residues | 17 |
| Details | SHMT Serine hydroxymethyltransferase pyridoxal-phosphate attachment site. HVvTTTTHKTLrGCRAG |
| Chain | Residue | Details |
| A | HIS221-GLY237 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | ACT_SITE: Nucleophile => ECO:0000305|PubMed:7358720 |
| Chain | Residue | Details |
| A | TYR177 | |
| B | TYR177 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | ACT_SITE: Proton donor => ECO:0000250 |
| Chain | Residue | Details |
| A | LYS229 | |
| B | LYS229 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | MOD_RES: N6-(pyridoxal phosphate)lysine => ECO:0000269|PubMed:10387080 |
| Chain | Residue | Details |
| A | THR230 | |
| B | THR230 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1dfo |
| Chain | Residue | Details |
| A | ARG63 |
| site_id | CSA2 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1dfo |
| Chain | Residue | Details |
| B | ARG63 |
| site_id | CSA3 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1dfo |
| Chain | Residue | Details |
| A | THR226 | |
| A | LYS229 | |
| A | GLU57 |
| site_id | CSA4 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1dfo |
| Chain | Residue | Details |
| B | THR226 | |
| B | LYS229 | |
| B | GLU57 |
| site_id | MCSA1 |
| Number of Residues | 6 |
| Details | M-CSA 147 |
| Chain | Residue | Details |
| A | SER56 | steric locator |
| A | GLY58 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor |
| A | MET201 | electrostatic stabiliser |
| A | THR227 | electrostatic stabiliser, hydrogen bond acceptor |
| A | THR230 | covalently attached, electron pair acceptor, electron pair donor, hydrogen bond donor, nucleofuge, nucleophile, proton acceptor, proton donor |
| A | ALA236 | electrostatic stabiliser |
| site_id | MCSA2 |
| Number of Residues | 6 |
| Details | M-CSA 147 |
| Chain | Residue | Details |
| B | SER56 | steric locator |
| B | GLY58 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor |
| B | MET201 | electrostatic stabiliser |
| B | THR227 | electrostatic stabiliser, hydrogen bond acceptor |
| B | THR230 | covalently attached, electron pair acceptor, electron pair donor, hydrogen bond donor, nucleofuge, nucleophile, proton acceptor, proton donor |
| B | ALA236 | electrostatic stabiliser |
