1CH0

RNASE T1 VARIANT WITH ALTERED GUANINE BINDING SEGMENT

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0001411	cellular_component	hyphal tip
A	0003723	molecular_function	RNA binding
A	0004519	molecular_function	endonuclease activity
A	0004521	molecular_function	RNA endonuclease activity
A	0004540	molecular_function	RNA nuclease activity
A	0008150	biological_process	biological_process
A	0016829	molecular_function	lyase activity
A	0030428	cellular_component	cell septum
A	0046589	molecular_function	ribonuclease T1 activity
B	0001411	cellular_component	hyphal tip
B	0003723	molecular_function	RNA binding
B	0004519	molecular_function	endonuclease activity
B	0004521	molecular_function	RNA endonuclease activity
B	0004540	molecular_function	RNA nuclease activity
B	0008150	biological_process	biological_process
B	0016829	molecular_function	lyase activity
B	0030428	cellular_component	cell septum
B	0046589	molecular_function	ribonuclease T1 activity
C	0001411	cellular_component	hyphal tip
C	0003723	molecular_function	RNA binding
C	0004519	molecular_function	endonuclease activity
C	0004521	molecular_function	RNA endonuclease activity
C	0004540	molecular_function	RNA nuclease activity
C	0008150	biological_process	biological_process
C	0016829	molecular_function	lyase activity
C	0030428	cellular_component	cell septum
C	0046589	molecular_function	ribonuclease T1 activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE CA A 107

Chain	Residue
A	GLU58
A	2GP105
A	HOH116
A	HOH117
A	HOH118
A	HOH119
A	HOH130

---

site_id	AC2
Number of Residues	2
Details	BINDING SITE FOR RESIDUE CA B 108

Chain	Residue
B	GLU58
B	HIS92

---

site_id	AC3
Number of Residues	7
Details	BINDING SITE FOR RESIDUE CA C 109

Chain	Residue
C	ASN36
C	2GP106
C	CL111
C	HOH122
C	HOH123
C	HOH124
C	HOH128

---

site_id	AC4
Number of Residues	7
Details	BINDING SITE FOR RESIDUE CA C 110

Chain	Residue
A	THR104
C	ASN36
C	2GP106
C	HOH143
C	HOH157
C	HOH158
C	HOH162

---

site_id	AC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CL C 111

Chain	Residue
A	ASN83
C	TYR38
C	GLU58
C	ARG77
C	HIS92
C	CA109

---

site_id	AC6
Number of Residues	16
Details	BINDING SITE FOR RESIDUE 2GP A 105

Chain	Residue
A	ASN36
A	HIS40
A	GLU41
A	PHE42
A	ARG43
A	ASN44
A	TRP45
A	ASN98
A	PHE100
A	CA107
A	HOH117
A	HOH119
A	HOH130
A	HOH132
A	HOH133
A	HOH162

---

site_id	AC7
Number of Residues	25
Details	BINDING SITE FOR RESIDUE 2GP C 106

Chain	Residue
A	THR5
A	SER53
A	THR104
A	HOH128
C	ASN36
C	HIS40
C	GLU41
C	PHE42
C	ARG43
C	ASN44
C	TRP45
C	ASN98
C	PHE100
C	CA109
C	CA110
C	HOH116
C	HOH123
C	HOH124
C	HOH132
C	HOH136
C	HOH138
C	HOH143
C	HOH148
C	HOH158
C	HOH164

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	3
Details	ACT_SITE: ACT_SITE => ECO:0000269|PubMed:2844811

Chain	Residue	Details
A	HIS40
B	HIS40
C	HIS40

---

site_id	SWS_FT_FI2
Number of Residues	3
Details	ACT_SITE: Proton acceptor => ECO:0000269|PubMed:2844811

Chain	Residue	Details
A	GLU58
B	GLU58
C	GLU58

---

site_id	SWS_FT_FI3
Number of Residues	3
Details	ACT_SITE: Proton donor

Chain	Residue	Details
A	HIS92
B	HIS92
C	HIS92

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	3
Details	Annotated By Reference To The Literature 1b2m

Chain	Residue	Details
A	GLU58
A	HIS40
A	HIS92

---

site_id	CSA2
Number of Residues	3
Details	Annotated By Reference To The Literature 1b2m

Chain	Residue	Details
B	GLU58
B	HIS40
B	HIS92

---

site_id	CSA3
Number of Residues	3
Details	Annotated By Reference To The Literature 1b2m

Chain	Residue	Details
C	GLU58
C	HIS40
C	HIS92

---

site_id	MCSA1
Number of Residues	6
Details	M-CSA 414

Chain	Residue	Details
A	TYR38	electrostatic stabiliser
A	HIS40	proton shuttle (general acid/base)
A	GLU58	proton shuttle (general acid/base)
A	ARG77	electrostatic stabiliser
A	HIS92	proton shuttle (general acid/base)
A	PHE100	electrostatic stabiliser

---

site_id	MCSA2
Number of Residues	6
Details	M-CSA 414

Chain	Residue	Details
B	TYR38	electrostatic stabiliser
B	HIS40	proton shuttle (general acid/base)
B	GLU58	proton shuttle (general acid/base)
B	ARG77	electrostatic stabiliser
B	HIS92	proton shuttle (general acid/base)
B	PHE100	electrostatic stabiliser

---

site_id	MCSA3
Number of Residues	6
Details	M-CSA 414

Chain	Residue	Details
C	TYR38	electrostatic stabiliser
C	HIS40	proton shuttle (general acid/base)
C	GLU58	proton shuttle (general acid/base)
C	ARG77	electrostatic stabiliser
C	HIS92	proton shuttle (general acid/base)
C	PHE100	electrostatic stabiliser

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