Functional Information from GO Data
Chain | GOid | namespace | contents |
A | 0004769 | molecular_function | steroid delta-isomerase activity |
A | 0005576 | cellular_component | extracellular region |
A | 0006707 | biological_process | cholesterol catabolic process |
A | 0008203 | biological_process | cholesterol metabolic process |
A | 0016491 | molecular_function | oxidoreductase activity |
A | 0016614 | molecular_function | oxidoreductase activity, acting on CH-OH group of donors |
A | 0016853 | molecular_function | isomerase activity |
A | 0016995 | molecular_function | cholesterol oxidase activity |
A | 0050660 | molecular_function | flavin adenine dinucleotide binding |
Functional Information from PDB Data
site_id | AC1 |
Number of Residues | 41 |
Details | BINDING SITE FOR RESIDUE FAD A 510 |
Chain | Residue |
A | GLU40 |
A | MET41 |
A | TYR107 |
A | ARG110 |
A | GLY111 |
A | GLY114 |
A | GLY115 |
A | ASN119 |
A | GLY120 |
A | GLY121 |
A | MET122 |
A | HIS248 |
A | GLN249 |
A | VAL250 |
A | GLY288 |
A | ALA289 |
A | GLY290 |
A | TYR446 |
A | ASN447 |
A | ASP474 |
A | GLY475 |
A | ASN485 |
A | PRO486 |
A | PHE487 |
A | ILE490 |
A | HOH517 |
A | HOH526 |
A | HOH529 |
A | HOH530 |
A | HOH532 |
A | HOH541 |
A | HOH550 |
A | HOH586 |
A | HOH599 |
A | HOH670 |
A | HOH783 |
A | GLY17 |
A | GLY19 |
A | TYR20 |
A | GLY21 |
A | LEU39 |
site_id | CAT |
Number of Residues | 4 |
Details | HISTIDINE 447 IS PRESUMED TO BE THE ACTIVE SITE BASE IN THE OXIDASE REACTION. THIS RESIDUE HAS BEEN MUTATED TO AN ASN. THE BASE CATALYZED MECHANISM IS MEDIATED THROUGH A WATER MOLECULE AT POSITION 541. GLU 361 IS THE BASE IN THE ISOMERIZATION REACTION. ASN 485 IS BELIEVED TO BE INVOLVED IN POSITIONING THE WATER MOLECULE. |
Chain | Residue |
A | ASN447 |
A | GLU361 |
A | ASN485 |
A | HOH541 |
Functional Information from PROSITE/UniProt
site_id | PS00623 |
Number of Residues | 24 |
Details | GMC_OXRED_1 GMC oxidoreductases signature 1. GRgVGGGSlVNggmAvePkrsyfE |
Chain | Residue | Details |
A | GLY109-GLU132 | |
Functional Information from SwissProt/UniProt
Chain | Residue | Details |
A | GLU361 | |
A | ASN447 | |
Chain | Residue | Details |
A | TYR20 | |
A | GLU40 | |
A | GLY115 | |
A | ASN119 | |
A | VAL250 | |
A | GLY475 | |
A | PHE487 | |
Chain | Residue | Details |
A | TYR446 | |
Catalytic Information from CSA
site_id | CSA1 |
Number of Residues | 3 |
Details | Annotated By Reference To The Literature 1coy |
Chain | Residue | Details |
A | ASN485 | |
A | GLU361 | |
A | ASN447 | |