1C0E
Active Site S19A Mutant of Bovine Heart Phosphotyrosyl Phosphatase
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003993 | molecular_function | acid phosphatase activity |
| A | 0004721 | molecular_function | phosphoprotein phosphatase activity |
| A | 0004725 | molecular_function | protein tyrosine phosphatase activity |
| A | 0004726 | molecular_function | non-membrane spanning protein tyrosine phosphatase activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0006470 | biological_process | protein dephosphorylation |
| A | 0016787 | molecular_function | hydrolase activity |
| B | 0003993 | molecular_function | acid phosphatase activity |
| B | 0004721 | molecular_function | phosphoprotein phosphatase activity |
| B | 0004725 | molecular_function | protein tyrosine phosphatase activity |
| B | 0004726 | molecular_function | non-membrane spanning protein tyrosine phosphatase activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0006470 | biological_process | protein dephosphorylation |
| B | 0016787 | molecular_function | hydrolase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE PO4 A 158 |
| Chain | Residue |
| A | CYS12 |
| A | LEU13 |
| A | GLY14 |
| A | ASN15 |
| A | ILE16 |
| A | CYS17 |
| A | ARG18 |
| A | ASP129 |
| site_id | AC2 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE PO4 B 358 |
| Chain | Residue |
| B | CYS212 |
| B | LEU213 |
| B | GLY214 |
| B | ASN215 |
| B | ILE216 |
| B | CYS217 |
| B | ARG218 |
| B | ASP329 |
| B | HOH65 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | Active site: {"description":"Nucleophile","evidences":[{"source":"PubMed","id":"8052313","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8132604","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8319676","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | Active site: {"evidences":[{"source":"PubMed","id":"8052313","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8319676","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | Active site: {"description":"Proton donor","evidences":[{"source":"PubMed","id":"8052313","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"UniProtKB","id":"P24666","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1pnt |
| Chain | Residue | Details |
| A | CYS17 | |
| A | ARG18 | |
| A | ASP129 | |
| A | CYS12 |
| site_id | CSA2 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1pnt |
| Chain | Residue | Details |
| B | CYS212 | |
| B | ARG218 | |
| B | ASP329 | |
| B | CYS217 |
| site_id | MCSA1 |
| Number of Residues | 6 |
| Details | M-CSA 462 |
| Chain | Residue | Details |
| A | CYS12 | covalent catalysis |
| A | ASN15 | activator, electrostatic stabiliser |
| A | CYS17 | steric role |
| A | ARG18 | transition state stabiliser |
| A | ALA19 | activator, electrostatic stabiliser |
| A | ASP129 | proton shuttle (general acid/base) |
| site_id | MCSA2 |
| Number of Residues | 6 |
| Details | M-CSA 462 |
| Chain | Residue | Details |
| B | CYS212 | covalent catalysis |
| B | ASN215 | activator, electrostatic stabiliser |
| B | CYS217 | steric role |
| B | ARG218 | transition state stabiliser |
| B | ALA219 | activator, electrostatic stabiliser |
| B | ASP329 | proton shuttle (general acid/base) |
