1BRM
ASPARTATE BETA-SEMIALDEHYDE DEHYDROGENASE FROM ESCHERICHIA COLI
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004073 | molecular_function | aspartate-semialdehyde dehydrogenase activity |
| A | 0005829 | cellular_component | cytosol |
| A | 0006520 | biological_process | amino acid metabolic process |
| A | 0006974 | biological_process | DNA damage response |
| A | 0008652 | biological_process | amino acid biosynthetic process |
| A | 0009067 | biological_process | aspartate family amino acid biosynthetic process |
| A | 0009088 | biological_process | L-threonine biosynthetic process |
| A | 0009089 | biological_process | L-lysine biosynthetic process via diaminopimelate |
| A | 0009090 | biological_process | L-homoserine biosynthetic process |
| A | 0009097 | biological_process | isoleucine biosynthetic process |
| A | 0016620 | molecular_function | oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor |
| A | 0019877 | biological_process | diaminopimelate biosynthetic process |
| A | 0046983 | molecular_function | protein dimerization activity |
| A | 0050661 | molecular_function | NADP binding |
| A | 0051287 | molecular_function | NAD binding |
| A | 0071266 | biological_process | 'de novo' L-methionine biosynthetic process |
| B | 0004073 | molecular_function | aspartate-semialdehyde dehydrogenase activity |
| B | 0005829 | cellular_component | cytosol |
| B | 0006520 | biological_process | amino acid metabolic process |
| B | 0006974 | biological_process | DNA damage response |
| B | 0008652 | biological_process | amino acid biosynthetic process |
| B | 0009067 | biological_process | aspartate family amino acid biosynthetic process |
| B | 0009088 | biological_process | L-threonine biosynthetic process |
| B | 0009089 | biological_process | L-lysine biosynthetic process via diaminopimelate |
| B | 0009090 | biological_process | L-homoserine biosynthetic process |
| B | 0009097 | biological_process | isoleucine biosynthetic process |
| B | 0016620 | molecular_function | oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor |
| B | 0019877 | biological_process | diaminopimelate biosynthetic process |
| B | 0046983 | molecular_function | protein dimerization activity |
| B | 0050661 | molecular_function | NADP binding |
| B | 0051287 | molecular_function | NAD binding |
| B | 0071266 | biological_process | 'de novo' L-methionine biosynthetic process |
| C | 0004073 | molecular_function | aspartate-semialdehyde dehydrogenase activity |
| C | 0005829 | cellular_component | cytosol |
| C | 0006520 | biological_process | amino acid metabolic process |
| C | 0006974 | biological_process | DNA damage response |
| C | 0008652 | biological_process | amino acid biosynthetic process |
| C | 0009067 | biological_process | aspartate family amino acid biosynthetic process |
| C | 0009088 | biological_process | L-threonine biosynthetic process |
| C | 0009089 | biological_process | L-lysine biosynthetic process via diaminopimelate |
| C | 0009090 | biological_process | L-homoserine biosynthetic process |
| C | 0009097 | biological_process | isoleucine biosynthetic process |
| C | 0016620 | molecular_function | oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor |
| C | 0019877 | biological_process | diaminopimelate biosynthetic process |
| C | 0046983 | molecular_function | protein dimerization activity |
| C | 0050661 | molecular_function | NADP binding |
| C | 0051287 | molecular_function | NAD binding |
| C | 0071266 | biological_process | 'de novo' L-methionine biosynthetic process |
Functional Information from PDB Data
| site_id | AT1 |
| Number of Residues | 4 |
| Details | ACTIVE SITE IDENTIFIED FROM MODIFIED CYS. |
| Chain | Residue |
| A | CYS135 |
| A | HIS274 |
| A | ARG267 |
| A | GLN162 |
| site_id | AT2 |
| Number of Residues | 4 |
| Details | ACTIVE SITE IDENTIFIED FROM MODIFIED CYS. |
| Chain | Residue |
| B | CYS135 |
| B | HIS274 |
| B | ARG267 |
| B | GLN162 |
| site_id | AT3 |
| Number of Residues | 4 |
| Details | ACTIVE SITE IDENTIFIED FROM MODIFIED CYS. |
| Chain | Residue |
| C | HIS274 |
| C | ARG267 |
| C | GLN162 |
| C | CYS135 |
Functional Information from PROSITE/UniProt
| site_id | PS01103 |
| Number of Residues | 15 |
| Details | ASD Aspartate-semialdehyde dehydrogenase signature. VDglCvRVgalrCHS |
| Chain | Residue | Details |
| A | VAL261-SER275 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 3 |
| Details | Active site: {"description":"Acyl-thioester intermediate","evidences":[{"source":"PubMed","id":"11724560","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 3 |
| Details | Active site: {"description":"Proton acceptor","evidences":[{"source":"PubMed","id":"11724560","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 36 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"11724560","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 6 |
| Details | Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_02121","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 7 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"11724560","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 3 |
| Details | Modified residue: {"description":"S-cysteinyl cysteine; in inhibited form"} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 3 |
| Details | a catalytic site defined by CSA, PubMed 1350921, 10369777 |
| Chain | Residue | Details |
| A | CYS135 | |
| A | HIS274 | |
| A | GLN162 |
| site_id | CSA2 |
| Number of Residues | 3 |
| Details | a catalytic site defined by CSA, PubMed 1350921, 10369777 |
| Chain | Residue | Details |
| B | CYS135 | |
| B | HIS274 | |
| B | GLN162 |
| site_id | CSA3 |
| Number of Residues | 3 |
| Details | a catalytic site defined by CSA, PubMed 1350921, 10369777 |
| Chain | Residue | Details |
| C | CYS135 | |
| C | HIS274 | |
| C | GLN162 |
| site_id | MCSA1 |
| Number of Residues | 4 |
| Details | M-CSA 429 |
| Chain | Residue | Details |
| A | CYS135 | covalent catalysis |
| A | GLN162 | electrostatic stabiliser |
| A | ARG267 | steric role |
| A | HIS274 | proton shuttle (general acid/base) |
| site_id | MCSA2 |
| Number of Residues | 4 |
| Details | M-CSA 429 |
| Chain | Residue | Details |
| B | CYS135 | covalent catalysis |
| B | GLN162 | electrostatic stabiliser |
| B | ARG267 | steric role |
| B | HIS274 | proton shuttle (general acid/base) |
| site_id | MCSA3 |
| Number of Residues | 4 |
| Details | M-CSA 429 |
| Chain | Residue | Details |
| C | CYS135 | covalent catalysis |
| C | GLN162 | electrostatic stabiliser |
| C | ARG267 | steric role |
| C | HIS274 | proton shuttle (general acid/base) |
