1BP6
THYMIDYLATE SYNTHASE R23I, R179T DOUBLE MUTANT
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004799 | molecular_function | thymidylate synthase activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0006231 | biological_process | dTMP biosynthetic process |
| A | 0006235 | biological_process | dTTP biosynthetic process |
| A | 0008168 | molecular_function | methyltransferase activity |
| A | 0009165 | biological_process | nucleotide biosynthetic process |
| A | 0016741 | molecular_function | transferase activity, transferring one-carbon groups |
| A | 0032259 | biological_process | methylation |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE K A 317 |
| Chain | Residue |
| A | SER183 |
| A | SER183 |
| A | TRP185 |
| A | TRP185 |
| site_id | AC2 |
| Number of Residues | 14 |
| Details | BINDING SITE FOR RESIDUE UMP A 318 |
| Chain | Residue |
| A | GLN217 |
| A | ARG218 |
| A | SER219 |
| A | ALA220 |
| A | ASP221 |
| A | GLY225 |
| A | ASN229 |
| A | HIS259 |
| A | TYR261 |
| A | HOH365 |
| A | ARG178 |
| A | LEU195 |
| A | CYS198 |
| A | HIS199 |
| site_id | CAT |
| Number of Residues | 1 |
| Details | CATALYTIC CYSTEINE |
| Chain | Residue |
| A | CYS198 |
Functional Information from PROSITE/UniProt
| site_id | PS00091 |
| Number of Residues | 29 |
| Details | THYMIDYLATE_SYNTHASE Thymidylate synthase active site. RtlIvsaWNpedvptma.....LpPCHtlyQFyV |
| Chain | Residue | Details |
| A | ARG178-VAL206 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | ACT_SITE: Nucleophile => ECO:0000255|HAMAP-Rule:MF_00008 |
| Chain | Residue | Details |
| A | CYS198 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | BINDING: in other chain => ECO:0000255|HAMAP-Rule:MF_00008 |
| Chain | Residue | Details |
| A | ASN229 | |
| A | HIS259 | |
| A | ILE23 | |
| A | ARG218 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 3 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00008 |
| Chain | Residue | Details |
| A | ASP221 | |
| A | ALA315 | |
| A | ARG178 |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 6 |
| Details | M-CSA 31 |
| Chain | Residue | Details |
| A | GLU60 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor |
| A | TRP82 | electrostatic stabiliser, hydrogen bond donor, van der waals interaction |
| A | TYR146 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor |
| A | CYS198 | covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor |
| A | ARG218 | electrostatic stabiliser, hydrogen bond donor, increase acidity |
| A | ASP221 | activator, electrostatic stabiliser, hydrogen bond acceptor, steric role |
