Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004553 | molecular_function | hydrolase activity, hydrolyzing O-glycosyl compounds |
| A | 0004556 | molecular_function | alpha-amylase activity |
| A | 0005509 | molecular_function | calcium ion binding |
| A | 0005576 | cellular_component | extracellular region |
| A | 0005975 | biological_process | carbohydrate metabolic process |
| A | 0016798 | molecular_function | hydrolase activity, acting on glycosyl bonds |
| A | 0046872 | molecular_function | metal ion binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE CA A 500 |
| Chain | Residue |
| A | ASP161 |
| A | ALA181 |
| A | ASP183 |
| A | ASP202 |
| A | ASP204 |
| A | HOH2079 |
| site_id | AC2 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE CA A 600 |
| Chain | Residue |
| A | ASN104 |
| A | ASP194 |
| A | ASP200 |
| A | HIS235 |
| A | HOH2080 |
| site_id | AC3 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE CA A 700 |
| Chain | Residue |
| A | GLY300 |
| A | TYR302 |
| A | HIS406 |
| A | ASP407 |
| A | ASP430 |
| A | HOH2069 |
| site_id | AC4 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE NA A 800 |
| Chain | Residue |
| A | ASP161 |
| A | ASP183 |
| A | ASP194 |
| A | ASP200 |
| A | ILE201 |
| site_id | CA1 |
| Number of Residues | 4 |
| Details | CALCIUM BINDING SITE. THIS CALCIUM BINDING SITE IS CONSERVED IN ALL STRUCTURES OF ALPHA-AMYLASES AND RELATED ENZYMES. |
| Chain | Residue |
| A | ASN104 |
| A | ASP194 |
| A | ASP200 |
| A | HIS235 |
| site_id | CA2 |
| Number of Residues | 5 |
| Details | CALCIUM BINDING SITE. THIS CALCIUM BINDING SITE IS NOT CONSERVED IN ALL STRUCTURES OF ALPHA-AMYLASES AND RELATED ENZYMES. |
| Chain | Residue |
| A | ASP183 |
| A | ASP202 |
| A | ASP204 |
| A | ASP161 |
| A | ALA181 |
| site_id | CA3 |
| Number of Residues | 5 |
| Details | CALCIUM BINDING SITE. THIS CALCIUM BINDING SITE LOCATED IN THE INTERFACE BETWEEN DOMAIN A AND C IS NOT CONSERVED IN ALL STRUCTURES OF ALPHA-AMYLASES AND RELATED ENZYMES. |
| Chain | Residue |
| A | GLY300 |
| A | TYR302 |
| A | HIS406 |
| A | ASP407 |
| A | ASP430 |
| site_id | CS |
| Number of Residues | 3 |
| Details | CATALYTIC SITE. |
| Chain | Residue |
| A | MET197 |
| A | VAL233 |
| A | GLY300 |
| site_id | NA1 |
| Number of Residues | 5 |
| Details | SODIUM BINDING SITE. THIS IS THE FIRST SODIUM BINDING SITE OBSERVED IN AN ALPHA-AMYLASE OR RELATED ENZYME. THIS SODIUM TOGETHER WITH THE CALCIUM IONS CA1 AND CA2 FORM A LINEAR TRIADIC ARRAY WITH THE CALCIUM IONS 4.1 AND 4.5 A FROM THE CENTRAL SODIUM ION. |
| Chain | Residue |
| A | ASP161 |
| A | ASP183 |
| A | ASP194 |
| A | ASP200 |
| A | ILE201 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | ACT_SITE: Nucleophile |
| Chain | Residue | Details |
| A | ASP231 | |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | ACT_SITE: Proton donor |
| Chain | Residue | Details |
| A | GLU261 | |
| Chain | Residue | Details |
| A | ASN104 | |
| A | ALA181 | |
| A | ASP202 | |
| A | ASP204 | |
| A | GLY300 | |
| A | TYR302 | |
| A | HIS406 | |
| A | ASP407 | |
| A | ASP430 | |
| Chain | Residue | Details |
| A | ASP161 | |
| A | ASP183 | |
| A | ASP194 | |
| A | ASP200 | |
| Chain | Residue | Details |
| A | HIS235 | |
| site_id | SWS_FT_FI6 |
| Number of Residues | 1 |
| Details | SITE: Transition state stabilizer => ECO:0000250 |
| Chain | Residue | Details |
| A | ASP328 | |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1amy |
| Chain | Residue | Details |
| A | ASP328 | |
| A | ASP231 | |
| A | GLU261 | |
| A | HIS105 | |
| site_id | CSA2 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1amy |
| Chain | Residue | Details |
| A | ASP328 | |
| A | ASP231 | |
| A | GLU261 | |
| site_id | CSA3 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1amy |
| Chain | Residue | Details |
| A | ASP231 | |
| A | GLU261 | |
| site_id | CSA4 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1amy |
| Chain | Residue | Details |
| A | ASP231 | |
| A | ASP328 | |
| A | TRP263 | |
| A | GLU261 | |
| site_id | CSA5 |
| Number of Residues | 5 |
| Details | Annotated By Reference To The Literature 1amy |
| Chain | Residue | Details |
| A | ASP231 | |
| A | ASP328 | |
| A | ARG229 | |
| A | GLU261 | |
| A | HIS327 | |
