1BK7

RIBONUCLEASE MC1 FROM THE SEEDS OF BITTER GOURD

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003723	molecular_function	RNA binding
A	0004519	molecular_function	endonuclease activity
A	0016829	molecular_function	lyase activity
A	0033897	molecular_function	ribonuclease T2 activity

Functional Information from PROSITE/UniProt

site_id	PS00530
Number of Residues	8
Details	RNASE_T2_1 Ribonuclease T2 family histidine active site 1. FtIHGLWP

Chain	Residue	Details
A	PHE31-PRO38

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site_id	PS00531
Number of Residues	12
Details	RNASE_T2_2 Ribonuclease T2 family histidine active site 2. FwsHEWtKHGtC

Chain	Residue	Details
A	PHE80-CYS91

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Proton donor => ECO:0000255|PROSITE-ProRule:PRU10045, ECO:0000269|PubMed:12731868, ECO:0000269|PubMed:15322360, ECO:0000305|PubMed:10446375, ECO:0007744|PDB:1J1F, ECO:0007744|PDB:1J1G, ECO:0007744|PDB:1UCD, ECO:0007744|PDB:1V9H

Chain	Residue	Details
A	HIS34

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site_id	SWS_FT_FI2
Number of Residues	1
Details	ACT_SITE: ACT_SITE => ECO:0000269|PubMed:10964705, ECO:0000269|PubMed:12731868, ECO:0000269|PubMed:15322360, ECO:0000305|PubMed:10446375, ECO:0007744|PDB:1J1F, ECO:0007744|PDB:1J1G, ECO:0007744|PDB:1UCA, ECO:0007744|PDB:1UCD, ECO:0007744|PDB:1V9H

Chain	Residue	Details
A	TRP85

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site_id	SWS_FT_FI3
Number of Residues	1
Details	ACT_SITE: Proton acceptor => ECO:0000255|PROSITE-ProRule:PRU10045, ECO:0000269|PubMed:12731868, ECO:0000269|PubMed:15322360, ECO:0000305|PubMed:10446375, ECO:0007744|PDB:1J1F, ECO:0007744|PDB:1J1G, ECO:0007744|PDB:1UCD, ECO:0007744|PDB:1V9H

Chain	Residue	Details
A	GLY89

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site_id	SWS_FT_FI4
Number of Residues	3
Details	BINDING: BINDING => ECO:0000305|PubMed:10964705, ECO:0000305|PubMed:12731868, ECO:0000305|PubMed:15322360, ECO:0007744|PDB:1J1F, ECO:0007744|PDB:1J1G, ECO:0007744|PDB:1UCA, ECO:0007744|PDB:1UCC, ECO:0007744|PDB:1UCD, ECO:0007744|PDB:1V9H

Chain	Residue	Details
A	GLN9
A	VAL72
A	HIS88

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site_id	SWS_FT_FI5
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269|PubMed:12731868, ECO:0000269|PubMed:15322360, ECO:0007744|PDB:1J1F, ECO:0007744|PDB:1J1G, ECO:0007744|PDB:1UCD, ECO:0007744|PDB:1V9H

Chain	Residue	Details
A	HIS34

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site_id	SWS_FT_FI6
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269|PubMed:15322360, ECO:0000305|PubMed:12731868, ECO:0007744|PDB:1J1F, ECO:0007744|PDB:1J1G, ECO:0007744|PDB:1V9H

Chain	Residue	Details
A	ALA75

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site_id	SWS_FT_FI7
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269|PubMed:15322360, ECO:0000305|PubMed:10964705, ECO:0000305|PubMed:12731868, ECO:0007744|PDB:1J1G, ECO:0007744|PDB:1UCA, ECO:0007744|PDB:1UCD, ECO:0007744|PDB:1V9H

Chain	Residue	Details
A	TRP81

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site_id	SWS_FT_FI8
Number of Residues	1
Details	BINDING: BINDING => ECO:0000305|PubMed:10964705, ECO:0000305|PubMed:12731868, ECO:0000305|PubMed:15322360, ECO:0007744|PDB:1J1F, ECO:0007744|PDB:1J1G, ECO:0007744|PDB:1UCA, ECO:0007744|PDB:1UCD, ECO:0007744|PDB:1V9H

Chain	Residue	Details
A	GLU84

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site_id	SWS_FT_FI9
Number of Residues	9
Details	SITE: Involved in thermostability => ECO:0000269|PubMed:15322360

Chain	Residue	Details
A	PHE102
A	LYS103
A	VAL106
A	ASN126
A	ARG128
A	LYS145
A	VAL163
A	VAL166

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Catalytic Information from CSA

site_id	CSA1
Number of Residues	3
Details	Annotated By Reference To The Literature 1bol

Chain	Residue	Details
A	HIS34
A	GLU84
A	HIS88

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