1BE0

HALOALKANE DEHALOGENASE AT PH 5.0 CONTAINING ACETIC ACID

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0009636	biological_process	response to toxic substance
A	0016787	molecular_function	hydrolase activity
A	0018786	molecular_function	haloalkane dehalogenase activity
A	0019260	biological_process	1,2-dichloroethane catabolic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	2
Details	BINDING SITE FOR RESIDUE ACT A 402

Chain	Residue
A	GLN10
A	ARG11

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site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE ACY A 401

Chain	Residue
A	GLU56
A	ASP124
A	TRP125
A	TRP175
A	PRO223
A	VAL226

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Nucleophile

Chain	Residue	Details
A	ASP124

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site_id	SWS_FT_FI2
Number of Residues	1
Details	ACT_SITE: Proton donor

Chain	Residue	Details
A	ASP260

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site_id	SWS_FT_FI3
Number of Residues	1
Details	ACT_SITE: Proton acceptor

Chain	Residue	Details
A	HIS289

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site_id	SWS_FT_FI4
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269|PubMed:10393294, ECO:0000269|PubMed:8515812

Chain	Residue	Details
A	TRP125

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site_id	SWS_FT_FI5
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269|PubMed:10393294, ECO:0000269|PubMed:8515812, ECO:0007744|PDB:2DHD, ECO:0007744|PDB:2DHE

Chain	Residue	Details
A	TRP175

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	5
Details	M-CSA 415

Chain	Residue	Details
A	ASP124	covalent catalysis
A	TRP125	electrostatic stabiliser
A	TRP175	electrostatic stabiliser
A	ASP260	activator, electrostatic stabiliser
A	HIS289	proton shuttle (general acid/base)

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