1B2M

THREE-DIMENSIONAL STRUCTURE OF RIBONULCEASE T1 COMPLEXED WITH AN ISOSTERIC PHOSPHONATE ANALOGUE OF GPU: ALTERNATE SUBSTRATE BINDING MODES AND CATALYSIS.

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0001411	cellular_component	hyphal tip
A	0003723	molecular_function	RNA binding
A	0004519	molecular_function	endonuclease activity
A	0004521	molecular_function	RNA endonuclease activity
A	0004540	molecular_function	RNA nuclease activity
A	0008150	biological_process	biological_process
A	0016829	molecular_function	lyase activity
A	0030428	cellular_component	cell septum
A	0046589	molecular_function	ribonuclease T1 activity
B	0001411	cellular_component	hyphal tip
B	0003723	molecular_function	RNA binding
B	0004519	molecular_function	endonuclease activity
B	0004521	molecular_function	RNA endonuclease activity
B	0004540	molecular_function	RNA nuclease activity
B	0008150	biological_process	biological_process
B	0016829	molecular_function	lyase activity
B	0030428	cellular_component	cell septum
B	0046589	molecular_function	ribonuclease T1 activity

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: ACT_SITE => ECO:0000269|PubMed:2844811

Chain	Residue	Details
A	HIS40
B	HIS40

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site_id	SWS_FT_FI2
Number of Residues	2
Details	ACT_SITE: Proton acceptor => ECO:0000269|PubMed:2844811

Chain	Residue	Details
A	GLU58
B	GLU58

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site_id	SWS_FT_FI3
Number of Residues	2
Details	ACT_SITE: Proton donor

Chain	Residue	Details
A	HIS92
B	HIS92

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Catalytic Information from CSA

site_id	CSA1
Number of Residues	6
Details	a catalytic site defined by CSA, PubMed 10029539, 20088566, 11048955

Chain	Residue	Details
A	TYR38
A	HIS40
A	GLU58
A	ARG77
A	HIS92
A	PHE100

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site_id	MCSA1
Number of Residues	6
Details	M-CSA 414

Chain	Residue	Details
A	TYR38	electrostatic stabiliser
A	HIS40	proton shuttle (general acid/base)
A	GLU58	proton shuttle (general acid/base)
A	ARG77	electrostatic stabiliser
A	HIS92	proton shuttle (general acid/base)
A	PHE100	electrostatic stabiliser

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site_id	MCSA2
Number of Residues	6
Details	M-CSA 414

Chain	Residue	Details
B	TYR38	electrostatic stabiliser
B	HIS40	proton shuttle (general acid/base)
B	GLU58	proton shuttle (general acid/base)
B	ARG77	electrostatic stabiliser
B	HIS92	proton shuttle (general acid/base)
B	PHE100	electrostatic stabiliser

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