Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

1AYZ

CRYSTAL STRUCTURE OF THE SACCHAROMYCES CEREVISIAE UBIQUITIN-CONJUGATING ENZYME RAD6 (UBC2) AT 2.6A RESOLUTION

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0000209	biological_process	protein polyubiquitination
A	0000722	biological_process	telomere maintenance via recombination
A	0000724	biological_process	double-strand break repair via homologous recombination
A	0000781	cellular_component	chromosome, telomeric region
A	0000785	cellular_component	chromatin
A	0003697	molecular_function	single-stranded DNA binding
A	0004842	molecular_function	ubiquitin-protein transferase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0006281	biological_process	DNA repair
A	0006325	biological_process	chromatin organization
A	0006351	biological_process	DNA-templated transcription
A	0006353	biological_process	DNA-templated transcription termination
A	0006366	biological_process	transcription by RNA polymerase II
A	0006511	biological_process	ubiquitin-dependent protein catabolic process
A	0006974	biological_process	DNA damage response
A	0009302	biological_process	sno(s)RNA transcription
A	0016567	biological_process	protein ubiquitination
A	0016740	molecular_function	transferase activity
A	0017116	molecular_function	single-stranded DNA helicase activity
A	0030435	biological_process	sporulation resulting in formation of a cellular spore
A	0031509	biological_process	subtelomeric heterochromatin formation
A	0031571	biological_process	mitotic G1 DNA damage checkpoint signaling
A	0033503	cellular_component	HULC complex
A	0034620	biological_process	cellular response to unfolded protein
A	0036503	biological_process	ERAD pathway
A	0042138	biological_process	meiotic DNA double-strand break formation
A	0042275	biological_process	error-free postreplication DNA repair
A	0042276	biological_process	error-prone translesion synthesis
A	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
A	0061631	molecular_function	ubiquitin conjugating enzyme activity
A	0070628	molecular_function	proteasome binding
A	0070987	biological_process	error-free translesion synthesis
A	0071596	biological_process	ubiquitin-dependent protein catabolic process via the N-end rule pathway
A	0071629	biological_process	cytoplasm protein quality control by the ubiquitin-proteasome system
A	0090089	biological_process	regulation of dipeptide transport
A	0097505	cellular_component	Rad6-Rad18 complex
A	0120174	biological_process	stress-induced homeostatically regulated protein degradation pathway
A	1990303	cellular_component	UBR1-RAD6 ubiquitin ligase complex
A	1990304	cellular_component	MUB1-RAD6-UBR2 ubiquitin ligase complex
B	0000166	molecular_function	nucleotide binding
B	0000209	biological_process	protein polyubiquitination
B	0000722	biological_process	telomere maintenance via recombination
B	0000724	biological_process	double-strand break repair via homologous recombination
B	0000781	cellular_component	chromosome, telomeric region
B	0000785	cellular_component	chromatin
B	0003697	molecular_function	single-stranded DNA binding
B	0004842	molecular_function	ubiquitin-protein transferase activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0006281	biological_process	DNA repair
B	0006325	biological_process	chromatin organization
B	0006351	biological_process	DNA-templated transcription
B	0006353	biological_process	DNA-templated transcription termination
B	0006366	biological_process	transcription by RNA polymerase II
B	0006511	biological_process	ubiquitin-dependent protein catabolic process
B	0006974	biological_process	DNA damage response
B	0009302	biological_process	sno(s)RNA transcription
B	0016567	biological_process	protein ubiquitination
B	0016740	molecular_function	transferase activity
B	0017116	molecular_function	single-stranded DNA helicase activity
B	0030435	biological_process	sporulation resulting in formation of a cellular spore
B	0031509	biological_process	subtelomeric heterochromatin formation
B	0031571	biological_process	mitotic G1 DNA damage checkpoint signaling
B	0033503	cellular_component	HULC complex
B	0034620	biological_process	cellular response to unfolded protein
B	0036503	biological_process	ERAD pathway
B	0042138	biological_process	meiotic DNA double-strand break formation
B	0042275	biological_process	error-free postreplication DNA repair
B	0042276	biological_process	error-prone translesion synthesis
B	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
B	0061631	molecular_function	ubiquitin conjugating enzyme activity
B	0070628	molecular_function	proteasome binding
B	0070987	biological_process	error-free translesion synthesis
B	0071596	biological_process	ubiquitin-dependent protein catabolic process via the N-end rule pathway
B	0071629	biological_process	cytoplasm protein quality control by the ubiquitin-proteasome system
B	0090089	biological_process	regulation of dipeptide transport
B	0097505	cellular_component	Rad6-Rad18 complex
B	0120174	biological_process	stress-induced homeostatically regulated protein degradation pathway
B	1990303	cellular_component	UBR1-RAD6 ubiquitin ligase complex
B	1990304	cellular_component	MUB1-RAD6-UBR2 ubiquitin ligase complex
C	0000166	molecular_function	nucleotide binding
C	0000209	biological_process	protein polyubiquitination
C	0000722	biological_process	telomere maintenance via recombination
C	0000724	biological_process	double-strand break repair via homologous recombination
C	0000781	cellular_component	chromosome, telomeric region
C	0000785	cellular_component	chromatin
C	0003697	molecular_function	single-stranded DNA binding
C	0004842	molecular_function	ubiquitin-protein transferase activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005634	cellular_component	nucleus
C	0005737	cellular_component	cytoplasm
C	0006281	biological_process	DNA repair
C	0006325	biological_process	chromatin organization
C	0006351	biological_process	DNA-templated transcription
C	0006353	biological_process	DNA-templated transcription termination
C	0006366	biological_process	transcription by RNA polymerase II
C	0006511	biological_process	ubiquitin-dependent protein catabolic process
C	0006974	biological_process	DNA damage response
C	0009302	biological_process	sno(s)RNA transcription
C	0016567	biological_process	protein ubiquitination
C	0016740	molecular_function	transferase activity
C	0017116	molecular_function	single-stranded DNA helicase activity
C	0030435	biological_process	sporulation resulting in formation of a cellular spore
C	0031509	biological_process	subtelomeric heterochromatin formation
C	0031571	biological_process	mitotic G1 DNA damage checkpoint signaling
C	0033503	cellular_component	HULC complex
C	0034620	biological_process	cellular response to unfolded protein
C	0036503	biological_process	ERAD pathway
C	0042138	biological_process	meiotic DNA double-strand break formation
C	0042275	biological_process	error-free postreplication DNA repair
C	0042276	biological_process	error-prone translesion synthesis
C	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
C	0061631	molecular_function	ubiquitin conjugating enzyme activity
C	0070628	molecular_function	proteasome binding
C	0070987	biological_process	error-free translesion synthesis
C	0071596	biological_process	ubiquitin-dependent protein catabolic process via the N-end rule pathway
C	0071629	biological_process	cytoplasm protein quality control by the ubiquitin-proteasome system
C	0090089	biological_process	regulation of dipeptide transport
C	0097505	cellular_component	Rad6-Rad18 complex
C	0120174	biological_process	stress-induced homeostatically regulated protein degradation pathway
C	1990303	cellular_component	UBR1-RAD6 ubiquitin ligase complex
C	1990304	cellular_component	MUB1-RAD6-UBR2 ubiquitin ligase complex

Functional Information from PDB Data

site_id	NUL
Number of Residues	1
Details	ACCEPTOR RESIDUE FOR ACTIVATED C-TERMINUS OF UBIQUITIN.

Chain	Residue
A	CYS88

Functional Information from PROSITE/UniProt

site_id	PS00183
Number of Residues	16
Details	UBC_1 Ubiquitin-conjugating (UBC) active site signature. FHPNVyan.GeICLdiL

Chain	Residue	Details
A	PHE77-LEU92

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	438
Details	Domain: {"description":"UBC core","evidences":[{"source":"PROSITE-ProRule","id":"PRU00388","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	3
Details	Active site: {"description":"Glycyl thioester intermediate","evidences":[{"source":"PROSITE-ProRule","id":"PRU00388","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"34789879","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	3
Details	Modified residue: {"description":"Phosphoserine; by SGV1","evidences":[{"source":"PubMed","id":"16307922","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"18407956","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	1
Details	M-CSA 939

Chain	Residue	Details
A	CYS88	nucleophile

site_id	MCSA2
Number of Residues	1
Details	M-CSA 939

Chain	Residue	Details
B	CYS88	nucleophile

site_id	MCSA3
Number of Residues	1
Details	M-CSA 939

Chain	Residue	Details
C	CYS88	nucleophile

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)