1APF
ANTHOPLEURIN-B, NMR, 20 STRUCTURES
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005576 | cellular_component | extracellular region |
| A | 0009966 | biological_process | regulation of signal transduction |
| A | 0017080 | molecular_function | sodium channel regulator activity |
| A | 0035821 | biological_process | modulation of process of another organism |
| A | 0042151 | cellular_component | nematocyst |
| A | 0090729 | molecular_function | toxin activity |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | SITE: Structurally important => ECO:0000305|PubMed:8639500 |
| Chain | Residue | Details |
| A | ASP7 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | SITE: Important for sodium channel affinity and for toxin structure => ECO:0000305|PubMed:8639500 |
| Chain | Residue | Details |
| A | ASP9 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | SITE: Important for affinity to sodium channel, probably due to the flexibility this residue gives to the Arg-14 loop => ECO:0000305|PubMed:14661964 |
| Chain | Residue | Details |
| A | GLY10 | |
| A | GLY15 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | SITE: Key residue for binding both cardiac and neuronal sodium channels => ECO:0000305|PubMed:8276803 |
| Chain | Residue | Details |
| A | ARG12 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 1 |
| Details | SITE: Important for sodium channel affinity => ECO:0000305|PubMed:8916901 |
| Chain | Residue | Details |
| A | PRO13 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 1 |
| Details | SITE: Not essential for sodium channel affinity => ECO:0000305|PubMed:8288644 |
| Chain | Residue | Details |
| A | ARG14 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 2 |
| Details | SITE: Binds to sodium channel => ECO:0000305|PubMed:15170345 |
| Chain | Residue | Details |
| A | ASN16 | |
| A | SER19 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 1 |
| Details | SITE: Has its side chain oriented away from the channel in the binary complex => ECO:0000305|PubMed:15170345 |
| Chain | Residue | Details |
| A | THR17 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 1 |
| Details | SITE: Important for high affinity to sodium channel => ECO:0000305|PubMed:8621610 |
| Chain | Residue | Details |
| A | LEU18 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 1 |
| Details | SITE: Structurally important => ECO:0000305|PubMed:14661964 |
| Chain | Residue | Details |
| A | GLY20 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 1 |
| Details | SITE: Important for channel affinity => ECO:0000305|PubMed:8798612 |
| Chain | Residue | Details |
| A | TRP33 |
| site_id | SWS_FT_FI12 |
| Number of Residues | 2 |
| Details | SITE: Not important for channel affinity and toxin structure => ECO:0000305|PubMed:8639500 |
| Chain | Residue | Details |
| A | HIS34 | |
| A | HIS39 |
| site_id | SWS_FT_FI13 |
| Number of Residues | 1 |
| Details | SITE: Important for channel affinity (interacts with rat Nav1.5 channel residue Asp-1612) => ECO:0000305|PubMed:8639500, ECO:0000305|PubMed:9417050 |
| Chain | Residue | Details |
| A | LYS37 |
| site_id | SWS_FT_FI14 |
| Number of Residues | 1 |
| Details | SITE: Structurally important => ECO:0000305|PubMed:8621610 |
| Chain | Residue | Details |
| A | ILE43 |
| site_id | SWS_FT_FI15 |
| Number of Residues | 1 |
| Details | SITE: Does not affect binding, but may affect the stabilization of the cardiac channel open conformation => ECO:0000305|PubMed:8798612 |
| Chain | Residue | Details |
| A | TRP45 |
| site_id | SWS_FT_FI16 |
| Number of Residues | 1 |
| Details | SITE: Binds to sodium channel => ECO:0000305|PubMed:8288644 |
| Chain | Residue | Details |
| A | LYS48 |
| site_id | SWS_FT_FI17 |
| Number of Residues | 1 |
| Details | SITE: Important for most of the cardiac specificity => ECO:0000305|PubMed:8276803 |
| Chain | Residue | Details |
| A | LYS49 |
