1AM4

COMPLEX BETWEEN CDC42HS.GMPPNP AND P50 RHOGAP (H. SAPIENS)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0007165	biological_process	signal transduction
B	0007165	biological_process	signal transduction
C	0007165	biological_process	signal transduction
D	0000139	cellular_component	Golgi membrane
D	0000322	cellular_component	storage vacuole
D	0002040	biological_process	sprouting angiogenesis
D	0003015	biological_process	heart process
D	0003161	biological_process	cardiac conduction system development
D	0003253	biological_process	cardiac neural crest cell migration involved in outflow tract morphogenesis
D	0003924	molecular_function	GTPase activity
D	0003925	molecular_function	G protein activity
D	0005515	molecular_function	protein binding
D	0005525	molecular_function	GTP binding
D	0005737	cellular_component	cytoplasm
D	0005789	cellular_component	endoplasmic reticulum membrane
D	0005813	cellular_component	centrosome
D	0005819	cellular_component	spindle
D	0005829	cellular_component	cytosol
D	0005856	cellular_component	cytoskeleton
D	0005886	cellular_component	plasma membrane
D	0005911	cellular_component	cell-cell junction
D	0005925	cellular_component	focal adhesion
D	0006897	biological_process	endocytosis
D	0006911	biological_process	phagocytosis, engulfment
D	0007015	biological_process	actin filament organization
D	0007030	biological_process	Golgi organization
D	0007088	biological_process	regulation of mitotic nuclear division
D	0007097	biological_process	nuclear migration
D	0007163	biological_process	establishment or maintenance of cell polarity
D	0007229	biological_process	integrin-mediated signaling pathway
D	0007264	biological_process	small GTPase-mediated signal transduction
D	0007399	biological_process	nervous system development
D	0008104	biological_process	protein localization
D	0009653	biological_process	anatomical structure morphogenesis
D	0010591	biological_process	regulation of lamellipodium assembly
D	0010592	biological_process	positive regulation of lamellipodium assembly
D	0016020	cellular_component	membrane
D	0016787	molecular_function	hydrolase activity
D	0017119	cellular_component	Golgi transport complex
D	0019901	molecular_function	protein kinase binding
D	0021762	biological_process	substantia nigra development
D	0030036	biological_process	actin cytoskeleton organization
D	0030141	cellular_component	secretory granule
D	0030154	biological_process	cell differentiation
D	0030175	cellular_component	filopodium
D	0030225	biological_process	macrophage differentiation
D	0030307	biological_process	positive regulation of cell growth
D	0030335	biological_process	positive regulation of cell migration
D	0030425	cellular_component	dendrite
D	0030496	cellular_component	midbody
D	0030742	molecular_function	GTP-dependent protein binding
D	0031252	cellular_component	cell leading edge
D	0031256	cellular_component	leading edge membrane
D	0031274	biological_process	positive regulation of pseudopodium assembly
D	0031333	biological_process	negative regulation of protein-containing complex assembly
D	0031435	molecular_function	mitogen-activated protein kinase kinase kinase binding
D	0031996	molecular_function	thioesterase binding
D	0032427	molecular_function	GBD domain binding
D	0032467	biological_process	positive regulation of cytokinesis
D	0032488	biological_process	Cdc42 protein signal transduction
D	0032956	biological_process	regulation of actin cytoskeleton organization
D	0032991	cellular_component	protein-containing complex
D	0034191	molecular_function	apolipoprotein A-I receptor binding
D	0034329	biological_process	cell junction assembly
D	0034332	biological_process	adherens junction organization
D	0035050	biological_process	embryonic heart tube development
D	0035088	biological_process	establishment or maintenance of apical/basal cell polarity
D	0036336	biological_process	dendritic cell migration
D	0036464	cellular_component	cytoplasmic ribonucleoprotein granule
D	0038189	biological_process	neuropilin signaling pathway
D	0042802	molecular_function	identical protein binding
D	0042995	cellular_component	cell projection
D	0043005	cellular_component	neuron projection
D	0043025	cellular_component	neuronal cell body
D	0043197	cellular_component	dendritic spine
D	0043227	cellular_component	membrane-bounded organelle
D	0043410	biological_process	positive regulation of MAPK cascade
D	0043525	biological_process	positive regulation of neuron apoptotic process
D	0044788	biological_process	modulation by host of viral process
D	0045177	cellular_component	apical part of cell
D	0045198	biological_process	establishment of epithelial cell apical/basal polarity
D	0045335	cellular_component	phagocytic vesicle
D	0045740	biological_process	positive regulation of DNA replication
D	0046330	biological_process	positive regulation of JNK cascade
D	0046847	biological_process	filopodium assembly
D	0048549	biological_process	positive regulation of pinocytosis
D	0048664	biological_process	neuron fate determination
D	0051130	biological_process	positive regulation of cellular component organization
D	0051233	cellular_component	spindle midzone
D	0051489	biological_process	regulation of filopodium assembly
D	0051491	biological_process	positive regulation of filopodium assembly
D	0051492	biological_process	regulation of stress fiber assembly
D	0051496	biological_process	positive regulation of stress fiber assembly
D	0051647	biological_process	nucleus localization
D	0051649	biological_process	establishment of localization in cell
D	0051683	biological_process	establishment of Golgi localization
D	0051835	biological_process	positive regulation of synapse structural plasticity
D	0051897	biological_process	positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction
D	0051988	biological_process	regulation of attachment of spindle microtubules to kinetochore
D	0060047	biological_process	heart contraction
D	0060071	biological_process	Wnt signaling pathway, planar cell polarity pathway
D	0060501	biological_process	positive regulation of epithelial cell proliferation involved in lung morphogenesis
D	0060661	biological_process	submandibular salivary gland formation
D	0060997	biological_process	dendritic spine morphogenesis
D	0061630	molecular_function	ubiquitin protein ligase activity
D	0070062	cellular_component	extracellular exosome
D	0071346	biological_process	cellular response to type II interferon
D	0071944	cellular_component	cell periphery
D	0072384	biological_process	organelle transport along microtubule
D	0072686	cellular_component	mitotic spindle
D	0086101	biological_process	endothelin receptor signaling pathway involved in heart process
D	0090135	biological_process	actin filament branching
D	0090316	biological_process	positive regulation of intracellular protein transport
D	0098685	cellular_component	Schaffer collateral - CA1 synapse
D	0098978	cellular_component	glutamatergic synapse
D	0099159	biological_process	regulation of modification of postsynaptic structure
D	0099175	biological_process	regulation of postsynapse organization
D	0099563	biological_process	modification of synaptic structure
D	1900026	biological_process	positive regulation of substrate adhesion-dependent cell spreading
E	0000139	cellular_component	Golgi membrane
E	0000322	cellular_component	storage vacuole
E	0002040	biological_process	sprouting angiogenesis
E	0003015	biological_process	heart process
E	0003161	biological_process	cardiac conduction system development
E	0003253	biological_process	cardiac neural crest cell migration involved in outflow tract morphogenesis
E	0003924	molecular_function	GTPase activity
E	0003925	molecular_function	G protein activity
E	0005515	molecular_function	protein binding
E	0005525	molecular_function	GTP binding
E	0005737	cellular_component	cytoplasm
E	0005789	cellular_component	endoplasmic reticulum membrane
E	0005813	cellular_component	centrosome
E	0005819	cellular_component	spindle
E	0005829	cellular_component	cytosol
E	0005856	cellular_component	cytoskeleton
E	0005886	cellular_component	plasma membrane
E	0005911	cellular_component	cell-cell junction
E	0005925	cellular_component	focal adhesion
E	0006897	biological_process	endocytosis
E	0006911	biological_process	phagocytosis, engulfment
E	0007015	biological_process	actin filament organization
E	0007030	biological_process	Golgi organization
E	0007088	biological_process	regulation of mitotic nuclear division
E	0007097	biological_process	nuclear migration
E	0007163	biological_process	establishment or maintenance of cell polarity
E	0007229	biological_process	integrin-mediated signaling pathway
E	0007264	biological_process	small GTPase-mediated signal transduction
E	0007399	biological_process	nervous system development
E	0008104	biological_process	protein localization
E	0009653	biological_process	anatomical structure morphogenesis
E	0010591	biological_process	regulation of lamellipodium assembly
E	0010592	biological_process	positive regulation of lamellipodium assembly
E	0016020	cellular_component	membrane
E	0016787	molecular_function	hydrolase activity
E	0017119	cellular_component	Golgi transport complex
E	0019901	molecular_function	protein kinase binding
E	0021762	biological_process	substantia nigra development
E	0030036	biological_process	actin cytoskeleton organization
E	0030141	cellular_component	secretory granule
E	0030154	biological_process	cell differentiation
E	0030175	cellular_component	filopodium
E	0030225	biological_process	macrophage differentiation
E	0030307	biological_process	positive regulation of cell growth
E	0030335	biological_process	positive regulation of cell migration
E	0030425	cellular_component	dendrite
E	0030496	cellular_component	midbody
E	0030742	molecular_function	GTP-dependent protein binding
E	0031252	cellular_component	cell leading edge
E	0031256	cellular_component	leading edge membrane
E	0031274	biological_process	positive regulation of pseudopodium assembly
E	0031333	biological_process	negative regulation of protein-containing complex assembly
E	0031435	molecular_function	mitogen-activated protein kinase kinase kinase binding
E	0031996	molecular_function	thioesterase binding
E	0032427	molecular_function	GBD domain binding
E	0032467	biological_process	positive regulation of cytokinesis
E	0032488	biological_process	Cdc42 protein signal transduction
E	0032956	biological_process	regulation of actin cytoskeleton organization
E	0032991	cellular_component	protein-containing complex
E	0034191	molecular_function	apolipoprotein A-I receptor binding
E	0034329	biological_process	cell junction assembly
E	0034332	biological_process	adherens junction organization
E	0035050	biological_process	embryonic heart tube development
E	0035088	biological_process	establishment or maintenance of apical/basal cell polarity
E	0036336	biological_process	dendritic cell migration
E	0036464	cellular_component	cytoplasmic ribonucleoprotein granule
E	0038189	biological_process	neuropilin signaling pathway
E	0042802	molecular_function	identical protein binding
E	0042995	cellular_component	cell projection
E	0043005	cellular_component	neuron projection
E	0043025	cellular_component	neuronal cell body
E	0043197	cellular_component	dendritic spine
E	0043227	cellular_component	membrane-bounded organelle
E	0043410	biological_process	positive regulation of MAPK cascade
E	0043525	biological_process	positive regulation of neuron apoptotic process
E	0044788	biological_process	modulation by host of viral process
E	0045177	cellular_component	apical part of cell
E	0045198	biological_process	establishment of epithelial cell apical/basal polarity
E	0045335	cellular_component	phagocytic vesicle
E	0045740	biological_process	positive regulation of DNA replication
E	0046330	biological_process	positive regulation of JNK cascade
E	0046847	biological_process	filopodium assembly
E	0048549	biological_process	positive regulation of pinocytosis
E	0048664	biological_process	neuron fate determination
E	0051130	biological_process	positive regulation of cellular component organization
E	0051233	cellular_component	spindle midzone
E	0051489	biological_process	regulation of filopodium assembly
E	0051491	biological_process	positive regulation of filopodium assembly
E	0051492	biological_process	regulation of stress fiber assembly
E	0051496	biological_process	positive regulation of stress fiber assembly
E	0051647	biological_process	nucleus localization
E	0051649	biological_process	establishment of localization in cell
E	0051683	biological_process	establishment of Golgi localization
E	0051835	biological_process	positive regulation of synapse structural plasticity
E	0051897	biological_process	positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction
E	0051988	biological_process	regulation of attachment of spindle microtubules to kinetochore
E	0060047	biological_process	heart contraction
E	0060071	biological_process	Wnt signaling pathway, planar cell polarity pathway
E	0060501	biological_process	positive regulation of epithelial cell proliferation involved in lung morphogenesis
E	0060661	biological_process	submandibular salivary gland formation
E	0060997	biological_process	dendritic spine morphogenesis
E	0061630	molecular_function	ubiquitin protein ligase activity
E	0070062	cellular_component	extracellular exosome
E	0071346	biological_process	cellular response to type II interferon
E	0071944	cellular_component	cell periphery
E	0072384	biological_process	organelle transport along microtubule
E	0072686	cellular_component	mitotic spindle
E	0086101	biological_process	endothelin receptor signaling pathway involved in heart process
E	0090135	biological_process	actin filament branching
E	0090316	biological_process	positive regulation of intracellular protein transport
E	0098685	cellular_component	Schaffer collateral - CA1 synapse
E	0098978	cellular_component	glutamatergic synapse
E	0099159	biological_process	regulation of modification of postsynaptic structure
E	0099175	biological_process	regulation of postsynapse organization
E	0099563	biological_process	modification of synaptic structure
E	1900026	biological_process	positive regulation of substrate adhesion-dependent cell spreading
F	0000139	cellular_component	Golgi membrane
F	0000322	cellular_component	storage vacuole
F	0002040	biological_process	sprouting angiogenesis
F	0003015	biological_process	heart process
F	0003161	biological_process	cardiac conduction system development
F	0003253	biological_process	cardiac neural crest cell migration involved in outflow tract morphogenesis
F	0003924	molecular_function	GTPase activity
F	0003925	molecular_function	G protein activity
F	0005515	molecular_function	protein binding
F	0005525	molecular_function	GTP binding
F	0005737	cellular_component	cytoplasm
F	0005789	cellular_component	endoplasmic reticulum membrane
F	0005813	cellular_component	centrosome
F	0005819	cellular_component	spindle
F	0005829	cellular_component	cytosol
F	0005856	cellular_component	cytoskeleton
F	0005886	cellular_component	plasma membrane
F	0005911	cellular_component	cell-cell junction
F	0005925	cellular_component	focal adhesion
F	0006897	biological_process	endocytosis
F	0006911	biological_process	phagocytosis, engulfment
F	0007015	biological_process	actin filament organization
F	0007030	biological_process	Golgi organization
F	0007088	biological_process	regulation of mitotic nuclear division
F	0007097	biological_process	nuclear migration
F	0007163	biological_process	establishment or maintenance of cell polarity
F	0007229	biological_process	integrin-mediated signaling pathway
F	0007264	biological_process	small GTPase-mediated signal transduction
F	0007399	biological_process	nervous system development
F	0008104	biological_process	protein localization
F	0009653	biological_process	anatomical structure morphogenesis
F	0010591	biological_process	regulation of lamellipodium assembly
F	0010592	biological_process	positive regulation of lamellipodium assembly
F	0016020	cellular_component	membrane
F	0016787	molecular_function	hydrolase activity
F	0017119	cellular_component	Golgi transport complex
F	0019901	molecular_function	protein kinase binding
F	0021762	biological_process	substantia nigra development
F	0030036	biological_process	actin cytoskeleton organization
F	0030141	cellular_component	secretory granule
F	0030154	biological_process	cell differentiation
F	0030175	cellular_component	filopodium
F	0030225	biological_process	macrophage differentiation
F	0030307	biological_process	positive regulation of cell growth
F	0030335	biological_process	positive regulation of cell migration
F	0030425	cellular_component	dendrite
F	0030496	cellular_component	midbody
F	0030742	molecular_function	GTP-dependent protein binding
F	0031252	cellular_component	cell leading edge
F	0031256	cellular_component	leading edge membrane
F	0031274	biological_process	positive regulation of pseudopodium assembly
F	0031333	biological_process	negative regulation of protein-containing complex assembly
F	0031435	molecular_function	mitogen-activated protein kinase kinase kinase binding
F	0031996	molecular_function	thioesterase binding
F	0032427	molecular_function	GBD domain binding
F	0032467	biological_process	positive regulation of cytokinesis
F	0032488	biological_process	Cdc42 protein signal transduction
F	0032956	biological_process	regulation of actin cytoskeleton organization
F	0032991	cellular_component	protein-containing complex
F	0034191	molecular_function	apolipoprotein A-I receptor binding
F	0034329	biological_process	cell junction assembly
F	0034332	biological_process	adherens junction organization
F	0035050	biological_process	embryonic heart tube development
F	0035088	biological_process	establishment or maintenance of apical/basal cell polarity
F	0036336	biological_process	dendritic cell migration
F	0036464	cellular_component	cytoplasmic ribonucleoprotein granule
F	0038189	biological_process	neuropilin signaling pathway
F	0042802	molecular_function	identical protein binding
F	0042995	cellular_component	cell projection
F	0043005	cellular_component	neuron projection
F	0043025	cellular_component	neuronal cell body
F	0043197	cellular_component	dendritic spine
F	0043227	cellular_component	membrane-bounded organelle
F	0043410	biological_process	positive regulation of MAPK cascade
F	0043525	biological_process	positive regulation of neuron apoptotic process
F	0044788	biological_process	modulation by host of viral process
F	0045177	cellular_component	apical part of cell
F	0045198	biological_process	establishment of epithelial cell apical/basal polarity
F	0045335	cellular_component	phagocytic vesicle
F	0045740	biological_process	positive regulation of DNA replication
F	0046330	biological_process	positive regulation of JNK cascade
F	0046847	biological_process	filopodium assembly
F	0048549	biological_process	positive regulation of pinocytosis
F	0048664	biological_process	neuron fate determination
F	0051130	biological_process	positive regulation of cellular component organization
F	0051233	cellular_component	spindle midzone
F	0051489	biological_process	regulation of filopodium assembly
F	0051491	biological_process	positive regulation of filopodium assembly
F	0051492	biological_process	regulation of stress fiber assembly
F	0051496	biological_process	positive regulation of stress fiber assembly
F	0051647	biological_process	nucleus localization
F	0051649	biological_process	establishment of localization in cell
F	0051683	biological_process	establishment of Golgi localization
F	0051835	biological_process	positive regulation of synapse structural plasticity
F	0051897	biological_process	positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction
F	0051988	biological_process	regulation of attachment of spindle microtubules to kinetochore
F	0060047	biological_process	heart contraction
F	0060071	biological_process	Wnt signaling pathway, planar cell polarity pathway
F	0060501	biological_process	positive regulation of epithelial cell proliferation involved in lung morphogenesis
F	0060661	biological_process	submandibular salivary gland formation
F	0060997	biological_process	dendritic spine morphogenesis
F	0061630	molecular_function	ubiquitin protein ligase activity
F	0070062	cellular_component	extracellular exosome
F	0071346	biological_process	cellular response to type II interferon
F	0071944	cellular_component	cell periphery
F	0072384	biological_process	organelle transport along microtubule
F	0072686	cellular_component	mitotic spindle
F	0086101	biological_process	endothelin receptor signaling pathway involved in heart process
F	0090135	biological_process	actin filament branching
F	0090316	biological_process	positive regulation of intracellular protein transport
F	0098685	cellular_component	Schaffer collateral - CA1 synapse
F	0098978	cellular_component	glutamatergic synapse
F	0099159	biological_process	regulation of modification of postsynaptic structure
F	0099175	biological_process	regulation of postsynapse organization
F	0099563	biological_process	modification of synaptic structure
F	1900026	biological_process	positive regulation of substrate adhesion-dependent cell spreading

Functional Information from PDB Data

site_id	AC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG D 679

Chain	Residue
D	THR517
D	THR535
D	GNP678

---

site_id	AC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG E 679

Chain	Residue
E	THR517
E	THR535
E	GNP678

---

site_id	AC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG F 679

Chain	Residue
F	THR517
F	THR535
F	GNP678

---

site_id	AC4
Number of Residues	22
Details	BINDING SITE FOR RESIDUE GNP D 678

Chain	Residue
D	HOH1
D	HOH66
D	HOH227
D	HOH274
D	GLY512
D	ALA513
D	VAL514
D	GLY515
D	LYS516
D	THR517
D	CYS518
D	TYR532
D	PRO534
D	THR535
D	GLY560
D	GLN561
D	GLN616
D	ASP618
D	LEU619
D	ALA659
D	LEU660
D	MG679

---

site_id	AC5
Number of Residues	20
Details	BINDING SITE FOR RESIDUE GNP E 678

Chain	Residue
E	HOH2
E	HOH71
E	GLY512
E	ALA513
E	VAL514
E	GLY515
E	LYS516
E	THR517
E	CYS518
E	TYR532
E	PRO534
E	THR535
E	GLY560
E	GLN561
E	GLN616
E	ASP618
E	LEU619
E	ALA659
E	LEU660
E	MG679

---

site_id	AC6
Number of Residues	20
Details	BINDING SITE FOR RESIDUE GNP F 678

Chain	Residue
F	HOH3
F	HOH96
F	GLY512
F	ALA513
F	VAL514
F	GLY515
F	LYS516
F	THR517
F	CYS518
F	TYR532
F	PRO534
F	THR535
F	GLY560
F	GLN561
F	GLN616
F	ASP618
F	LEU619
F	ALA659
F	LEU660
F	MG679

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	6
Details	BINDING:

Chain	Residue	Details
D	GLY510
D	THR615
E	GLY510
E	THR615
F	GLY510
F	THR615

---

site_id	SWS_FT_FI2
Number of Residues	3
Details	BINDING: BINDING => ECO:0000250

Chain	Residue	Details
D	ASP557
E	ASP557
F	ASP557

---

site_id	SWS_FT_FI3
Number of Residues	3
Details	MOD_RES: (Microbial infection) O-AMP-tyrosine; by Haemophilus IbpA; alternate => ECO:0000269|PubMed:19362538, ECO:0000269|PubMed:20622875

Chain	Residue	Details
D	TYR532
E	TYR532
F	TYR532

---

site_id	SWS_FT_FI4
Number of Residues	3
Details	MOD_RES: (Microbial infection) O-AMP-threonine; by Vibrio VopS => ECO:0000269|PubMed:19039103

Chain	Residue	Details
D	THR535
E	THR535
F	THR535

---

site_id	SWS_FT_FI5
Number of Residues	3
Details	MOD_RES: Phosphotyrosine; by SRC => ECO:0000269|PubMed:14506284

Chain	Residue	Details
D	TYR564
E	TYR564
F	TYR564

---

site_id	SWS_FT_FI6
Number of Residues	3
Details	CARBOHYD: (Microbial infection) O-linked (GlcNAc) tyrosine; by Photorhabdus PAU_02230; alternate => ECO:0000269|PubMed:24141704

Chain	Residue	Details
D	TYR532
E	TYR532
F	TYR532

---

site_id	SWS_FT_FI7
Number of Residues	3
Details	CARBOHYD: (Microbial infection) O-linked (Glc) threonine; by C.difficile toxins TcdA and TcdB; alternate => ECO:0000269|PubMed:24905543, ECO:0000269|PubMed:7775453, ECO:0000269|PubMed:7777059

Chain	Residue	Details
D	THR535
E	THR535
F	THR535

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	1
Details	Annotated By Reference To The Literature 1ksj

Chain	Residue	Details
D	GLN561

---

site_id	CSA2
Number of Residues	1
Details	Annotated By Reference To The Literature 1ksj

Chain	Residue	Details
E	GLN561

---

site_id	CSA3
Number of Residues	1
Details	Annotated By Reference To The Literature 1ksj

Chain	Residue	Details
F	GLN561

---