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1AGR

COMPLEX OF ALF4-ACTIVATED GI-ALPHA-1 WITH RGS4

Functional Information from GO Data
ChainGOidnamespacecontents
A0000287molecular_functionmagnesium ion binding
A0001664molecular_functionG protein-coupled receptor binding
A0003924molecular_functionGTPase activity
A0005515molecular_functionprotein binding
A0005525molecular_functionGTP binding
A0005634cellular_componentnucleus
A0005737cellular_componentcytoplasm
A0005813cellular_componentcentrosome
A0005834cellular_componentheterotrimeric G-protein complex
A0005856cellular_componentcytoskeleton
A0005886cellular_componentplasma membrane
A0005938cellular_componentcell cortex
A0007165biological_processsignal transduction
A0007186biological_processG protein-coupled receptor signaling pathway
A0007188biological_processadenylate cyclase-modulating G protein-coupled receptor signaling pathway
A0016787molecular_functionhydrolase activity
A0019001molecular_functionguanyl nucleotide binding
A0019003molecular_functionGDP binding
A0030496cellular_componentmidbody
A0031683molecular_functionG-protein beta/gamma-subunit complex binding
A0031749molecular_functionD2 dopamine receptor binding
A0031821molecular_functionG protein-coupled serotonin receptor binding
A0032794molecular_functionGTPase activating protein binding
A0032991cellular_componentprotein-containing complex
A0043949biological_processregulation of cAMP-mediated signaling
A0046872molecular_functionmetal ion binding
A0050805biological_processnegative regulation of synaptic transmission
A0051301biological_processcell division
A0060236biological_processregulation of mitotic spindle organization
A0098794cellular_componentpostsynapse
A0098978cellular_componentglutamatergic synapse
A0099645biological_processneurotransmitter receptor localization to postsynaptic specialization membrane
A1904322biological_processcellular response to forskolin
A1904778biological_processpositive regulation of protein localization to cell cortex
D0000287molecular_functionmagnesium ion binding
D0001664molecular_functionG protein-coupled receptor binding
D0003924molecular_functionGTPase activity
D0005515molecular_functionprotein binding
D0005525molecular_functionGTP binding
D0005634cellular_componentnucleus
D0005737cellular_componentcytoplasm
D0005813cellular_componentcentrosome
D0005834cellular_componentheterotrimeric G-protein complex
D0005856cellular_componentcytoskeleton
D0005886cellular_componentplasma membrane
D0005938cellular_componentcell cortex
D0007165biological_processsignal transduction
D0007186biological_processG protein-coupled receptor signaling pathway
D0007188biological_processadenylate cyclase-modulating G protein-coupled receptor signaling pathway
D0016787molecular_functionhydrolase activity
D0019001molecular_functionguanyl nucleotide binding
D0019003molecular_functionGDP binding
D0030496cellular_componentmidbody
D0031683molecular_functionG-protein beta/gamma-subunit complex binding
D0031749molecular_functionD2 dopamine receptor binding
D0031821molecular_functionG protein-coupled serotonin receptor binding
D0032794molecular_functionGTPase activating protein binding
D0032991cellular_componentprotein-containing complex
D0043949biological_processregulation of cAMP-mediated signaling
D0046872molecular_functionmetal ion binding
D0050805biological_processnegative regulation of synaptic transmission
D0051301biological_processcell division
D0060236biological_processregulation of mitotic spindle organization
D0098794cellular_componentpostsynapse
D0098978cellular_componentglutamatergic synapse
D0099645biological_processneurotransmitter receptor localization to postsynaptic specialization membrane
D1904322biological_processcellular response to forskolin
D1904778biological_processpositive regulation of protein localization to cell cortex
E0001965molecular_functionG-protein alpha-subunit binding
E0001975biological_processresponse to amphetamine
E0005096molecular_functionGTPase activator activity
E0005634cellular_componentnucleus
E0005737cellular_componentcytoplasm
E0009968biological_processnegative regulation of signal transduction
E0010460biological_processpositive regulation of heart rate
E0019901molecular_functionprotein kinase binding
E0032991cellular_componentprotein-containing complex
E0042220biological_processresponse to cocaine
E0045471biological_processresponse to ethanol
E0045744biological_processnegative regulation of G protein-coupled receptor signaling pathway
E0051924biological_processregulation of calcium ion transport
E0060160biological_processnegative regulation of dopamine receptor signaling pathway
E0061052biological_processnegative regulation of cell growth involved in cardiac muscle cell development
E0110053biological_processregulation of actin filament organization
E1900924biological_processnegative regulation of glycine import across plasma membrane
E1901379biological_processregulation of potassium ion transmembrane transport
E1901380biological_processnegative regulation of potassium ion transmembrane transport
E1990791biological_processdorsal root ganglion development
E2000463biological_processpositive regulation of excitatory postsynaptic potential
H0001965molecular_functionG-protein alpha-subunit binding
H0001975biological_processresponse to amphetamine
H0005096molecular_functionGTPase activator activity
H0005634cellular_componentnucleus
H0005737cellular_componentcytoplasm
H0009968biological_processnegative regulation of signal transduction
H0010460biological_processpositive regulation of heart rate
H0019901molecular_functionprotein kinase binding
H0032991cellular_componentprotein-containing complex
H0042220biological_processresponse to cocaine
H0045471biological_processresponse to ethanol
H0045744biological_processnegative regulation of G protein-coupled receptor signaling pathway
H0051924biological_processregulation of calcium ion transport
H0060160biological_processnegative regulation of dopamine receptor signaling pathway
H0061052biological_processnegative regulation of cell growth involved in cardiac muscle cell development
H0110053biological_processregulation of actin filament organization
H1900924biological_processnegative regulation of glycine import across plasma membrane
H1901379biological_processregulation of potassium ion transmembrane transport
H1901380biological_processnegative regulation of potassium ion transmembrane transport
H1990791biological_processdorsal root ganglion development
H2000463biological_processpositive regulation of excitatory postsynaptic potential
Functional Information from PDB Data
site_idAC1
Number of Residues6
DetailsBINDING SITE FOR RESIDUE MG A 356
ChainResidue
ASER47
ATHR181
AGDP355
AALF357
AHOH364
AHOH365

site_idAC2
Number of Residues15
DetailsBINDING SITE FOR RESIDUE ALF A 357
ChainResidue
AARG178
ALYS180
ATHR181
AVAL201
AGLY202
AGLY203
AGLN204
AGDP355
AMG356
AHOH359
AHOH364
AHOH365
AGLY42
AGLU43
ALYS46

site_idAC3
Number of Residues6
DetailsBINDING SITE FOR RESIDUE MG D 356
ChainResidue
DSER47
DTHR181
DGDP355
DALF357
DHOH365
DHOH366

site_idAC4
Number of Residues15
DetailsBINDING SITE FOR RESIDUE ALF D 357
ChainResidue
DGLY42
DGLU43
DLYS46
DARG178
DLYS180
DTHR181
DVAL201
DGLY202
DGLY203
DGLN204
DGDP355
DMG356
DHOH360
DHOH365
DHOH366

site_idAC5
Number of Residues24
DetailsBINDING SITE FOR RESIDUE GDP A 355
ChainResidue
AGLU43
ASER44
AGLY45
ALYS46
ASER47
ATHR48
ASER151
ALEU175
AARG176
ATHR177
AARG178
AASN269
ALYS270
AASP272
ALEU273
ACYS325
AALA326
ATHR327
AMG356
AALF357
AHOH360
AHOH361
AHOH364
AHOH365

site_idAC6
Number of Residues11
DetailsBINDING SITE FOR RESIDUE CIT A 358
ChainResidue
AGLU275
ATHR295
ATYR296
AGLU297
AHOH384
DLYS271
DHIS322
DPHE323
DASN331
DPHE334
DHOH384

site_idAC7
Number of Residues24
DetailsBINDING SITE FOR RESIDUE GDP D 355
ChainResidue
DHOH365
DHOH366
DGLU43
DSER44
DGLY45
DLYS46
DSER47
DTHR48
DSER151
DLEU175
DARG176
DTHR177
DARG178
DASN269
DLYS270
DASP272
DLEU273
DCYS325
DALA326
DTHR327
DMG356
DALF357
DHOH361
DHOH362

site_idAC8
Number of Residues9
DetailsBINDING SITE FOR RESIDUE CIT D 358
ChainResidue
ALYS271
AHIS322
APHE323
AASN331
DGLU275
DTHR295
DTYR296
DGLU297
DHOH359

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues6
DetailsLIPID: S-palmitoyl cysteine => ECO:0000250
ChainResidueDetails
ECYS2
ECYS12
ECYS95
HCYS2
HCYS12
HCYS95
DVAL201
DLYS270

site_idSWS_FT_FI2
Number of Residues2
DetailsBINDING: BINDING => ECO:0000269|PubMed:25037222, ECO:0000269|PubMed:9705312, ECO:0007744|PDB:1BH2, ECO:0007744|PDB:4PAO
ChainResidueDetails
ATHR48
DTHR48

site_idSWS_FT_FI3
Number of Residues2
DetailsBINDING: BINDING => ECO:0007744|PDB:1AS0, ECO:0007744|PDB:1BH2, ECO:0007744|PDB:1GIL, ECO:0007744|PDB:4N0D
ChainResidueDetails
ASER151
DSER151

site_idSWS_FT_FI4
Number of Residues2
DetailsBINDING: BINDING => ECO:0000269|PubMed:19703466, ECO:0000269|PubMed:25037222, ECO:0000269|PubMed:9705312, ECO:0007744|PDB:1BH2, ECO:0007744|PDB:4PAO
ChainResidueDetails
ATHR182
DTHR182

site_idSWS_FT_FI5
Number of Residues2
DetailsBINDING: BINDING => ECO:0007744|PDB:1AS0, ECO:0007744|PDB:1GIA, ECO:0007744|PDB:1GIL, ECO:0007744|PDB:3FFA, ECO:0007744|PDB:4N0D, ECO:0007744|PDB:4PAO, ECO:0007744|PDB:4PAQ
ChainResidueDetails
ATHR327
DTHR327

site_idSWS_FT_FI6
Number of Residues2
DetailsLIPID: N-myristoyl glycine => ECO:0000269|PubMed:26253820
ChainResidueDetails
ACYS3
DCYS3

site_idSWS_FT_FI7
Number of Residues2
DetailsLIPID: S-palmitoyl cysteine => ECO:0000269|PubMed:26253820
ChainResidueDetails
ATHR4
DTHR4

Catalytic Information from CSA
site_idCSA1
Number of Residues4
DetailsAnnotated By Reference To The Literature 1ksj
ChainResidueDetails
ATHR181
AGLN204
AGLU43
AARG178

site_idCSA2
Number of Residues4
DetailsAnnotated By Reference To The Literature 1ksj
ChainResidueDetails
DTHR181
DGLN204
DGLU43
DARG178

site_idCSA3
Number of Residues1
DetailsAnnotated By Reference To The Literature 1ksj
ChainResidueDetails
AGLN204

site_idCSA4
Number of Residues1
DetailsAnnotated By Reference To The Literature 1ksj
ChainResidueDetails
DGLN204

site_idMCSA1
Number of Residues5
DetailsM-CSA 533
ChainResidueDetails
ASER44electrostatic stabiliser
AILE49electrostatic stabiliser
AVAL179electrostatic stabiliser
AVAL201electrostatic stabiliser
AARG205electrostatic stabiliser

site_idMCSA2
Number of Residues5
DetailsM-CSA 533
ChainResidueDetails
DSER44electrostatic stabiliser
DILE49electrostatic stabiliser
DVAL179electrostatic stabiliser
DVAL201electrostatic stabiliser
DARG205electrostatic stabiliser

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PDB entries from 2024-10-30

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