1A80

Native 2,5-DIKETO-D-GLUCONIC acid reductase a from CORYNBACTERIUM SP. complexed with nadph

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005737	cellular_component	cytoplasm
A	0016491	molecular_function	oxidoreductase activity
A	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
A	0019853	biological_process	L-ascorbic acid biosynthetic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	31
Details	BINDING SITE FOR RESIDUE NDP A 300

Chain	Residue
A	GLY20
A	VAL21
A	PHE22
A	ASP45
A	TYR50
A	LYS75
A	HIS108
A	SER139
A	ASN140
A	GLN161
A	TRP187
A	GLY188
A	PRO189
A	LEU190
A	GLY191
A	GLN192
A	ALA215
A	PHE230
A	PRO231
A	LYS232
A	SER233
A	VAL234
A	ARG235
A	ARG238
A	GLU241
A	ASN242
A	HOH332
A	HOH342
A	HOH352
A	HOH396
A	HOH406

---

site_id	CIC
Number of Residues	9
Details	THE RESIDUE LINE THE ACTIVE SITE OF THE ENZYME.

Chain	Residue
A	PHE22
A	ASP45
A	ALA47
A	TYR50
A	LYS75
A	LEU106
A	SER139
A	ASN140
A	TRP187

---

Functional Information from PROSITE/UniProt

site_id	PS00798
Number of Residues	18
Details	ALDOKETO_REDUCTASE_1 Aldo/keto reductase family signature 1. GYRHIDTAaiygnEegVG

Chain	Residue	Details
A	GLY40-GLY57

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Proton donor => ECO:0000269|PubMed:11399090

Chain	Residue	Details
A	TYR50

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269|PubMed:11399090

Chain	Residue	Details
A	HIS108

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269|PubMed:14718658

Chain	Residue	Details
A	GLY188

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	4
Details	Annotated By Reference To The Literature 1mrq

Chain	Residue	Details
A	LYS75
A	ASP45
A	TYR50
A	HIS108

---

site_id	CSA2
Number of Residues	2
Details	Annotated By Reference To The Literature 1mrq

Chain	Residue	Details
A	LYS75
A	TYR50

---