1A2U

STAPHYLOCOCCAL NUCLEASE, V23C VARIANT, COMPLEX WITH 1-N-BUTANE THIOL AND 3',5'-THYMIDINE DIPHOSPHATE

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003676	molecular_function	nucleic acid binding
A	0004518	molecular_function	nuclease activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE CA A 150

Chain	Residue
A	ASP21
A	ASP40
A	THR41
A	THP151
A	HOH212
A	HOH217
A	HOH243

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site_id	AC2
Number of Residues	16
Details	BINDING SITE FOR RESIDUE THP A 151

Chain	Residue
A	ASP40
A	LYS71
A	ASP83
A	LYS84
A	TYR85
A	ARG87
A	TYR113
A	TYR115
A	CA150
A	HOH202
A	HOH212
A	HOH215
A	HOH216
A	HOH243
A	ARG35
A	LEU36

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Functional Information from PROSITE/UniProt

site_id	PS01284
Number of Residues	11
Details	TNASE_2 Thermonuclease family signature 2. DKYGRgLAyIY

Chain	Residue	Details
A	ASP83-TYR93

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	16
Details	Compositional bias: {"description":"Basic and acidic residues","evidences":[{"source":"SAM","id":"MobiDB-lite","evidenceCode":"ECO:0000256"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI2
Number of Residues	3
Details	Active site: {"evidences":[{"source":"PubMed","id":"288045","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI3
Number of Residues	3
Details	Binding site: {}

Chain

Residue

Details

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Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1a2t

Chain	Residue	Details
A	ARG35
A	ARG87

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site_id	MCSA1
Number of Residues	6
Details	M-CSA 165

Chain	Residue	Details
A	ASP21	metal ligand
A	VAL39	electrostatic stabiliser, hydrogen bond donor
A	THR44	metal ligand
A	LYS45	metal ligand
A	PRO47	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
A	TYR91	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor

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