9ZUM

Structure of the Caulobacter crescentus RNAP alpha-CTD bound to AT rich DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-10-03
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	1.0
Spacegroup name	P 41
Unit cell lengths	153.277, 153.277, 32.078
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.510 - 2.430
R-factor	0.213
Rwork	0.211
R-free	0.24160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.711
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.470	2.520
High resolution limit [Å]	2.430	2.430
Rpim	0.034	0.471
Number of reflections	27074	1493
<I/σ(I)>	23.6	1.5
Completeness [%]	93.2
Redundancy	5.6
CC(1/2)	0.999	0.593

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1-2% PEG 2000 MME, 1 mM Ammonium sulphate, 20 mM sodium acetate pH 5.5