9ZPG

Crystal structure of Glutamate--tRNA ligase (GltX) from Moraxella catarrhalis (Apo)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 19-ID
Synchrotron site	NSLS-II
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-10-04
Detector	DECTRIS EIGER2 XE 9M
Wavelength(s)	0.9786
Spacegroup name	P 1 21 1
Unit cell lengths	103.557, 53.602, 127.962
Unit cell angles	90.00, 108.19, 90.00

Refinement procedure

Resolution	47.070 - 2.750
R-factor	0.2177
Rwork	0.215
R-free	0.26130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.587
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((2.0_5904: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.070	2.880
High resolution limit [Å]	2.750	2.750
Rmerge	0.147	1.310
Rmeas	0.160	1.410
Rpim	0.060	0.519
Total number of observations	239953	33376
Number of reflections	35110	4621
<I/σ(I)>	10	1.5
Completeness [%]	99.6
Redundancy	6.8	7.2
CC(1/2)	0.997	0.634

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	Grid Salt HT B10: 3.0M Ammonium Sulfate 0.1M HEPES pH 7.0, MocaA.01348.a.UX11.PS38771 at 26.6 mg/mL. plate 20153 B10 drop 2, Puck: PSL-1810, Cryo: 2.5 M ammonium sulfate.