9ZNB

Crystal Structure of a DARPin Fused to the 1TEL Crystallization Chaperone via a Direct Helical Fusion in a 3-Fold Crystal Form

Replaces:  9DAM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-04-06
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.979460
Spacegroup name	P 65
Unit cell lengths	83.286, 83.286, 58.431
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.640 - 1.771
R-factor	0.1954
Rwork	0.194
R-free	0.21950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	0.978
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.640	1.835
High resolution limit [Å]	1.771	1.771
Rmerge	0.149	6.504
Rmeas	0.153	6.676
Rpim	0.034	1.490
Number of reflections	21254	1434
<I/σ(I)>	13.52	0.54
Completeness [%]	90.4	64.57
Redundancy	20.3	19.6
CC(1/2)	0.998	0.340

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.6	294	1.0 M Magnesium sulfate hydrate, 0.1 M Sodium acetate trihydrate pH 4.6