9ZMB

Crystal structure of Fab 7118 in complex with minor repeat region from circumsporozoite protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-03-20
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	73.840, 83.037, 88.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.940 - 2.090
R-factor	0.2089
Rwork	0.207
R-free	0.24260
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.572
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.140
High resolution limit [Å]	2.090	2.090
Number of reflections	30491	1561
<I/σ(I)>	29
Completeness [%]	94.0
Redundancy	5.1
CC(1/2)	0.990	0.860

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M HEPES (pH 7.5), 30% (v/v) 1,2-propanediol, and 20% (v/v) PEG 400