9ZMA

Crystal structure of Fab 7160 in complex with major repeat region (NANP6)from circumsporozoite protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-04-05
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92
Spacegroup name	P 1 21 1
Unit cell lengths	103.213, 73.131, 134.373
Unit cell angles	90.00, 102.25, 90.00

Refinement procedure

Resolution	41.740 - 2.330
R-factor	0.2074
Rwork	0.207
R-free	0.23610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.544
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.390
High resolution limit [Å]	2.330	2.330
Number of reflections	82863	4058
<I/σ(I)>	13.1
Completeness [%]	99.1
Redundancy	6.6
CC(1/2)	0.980	0.890

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M magnesium nitrate (pH 5.8) and 20% (w/v) PEG 3350.