9ZK0
Crystal structure of a calcium bound C2 domain containing protein from Trichomonas vaginalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 19-ID |
| Synchrotron site | NSLS-II |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-09-20 |
| Detector | DECTRIS EIGER2 XE 9M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 1 |
| Unit cell lengths | 25.329, 50.197, 56.300 |
| Unit cell angles | 74.23, 82.17, 79.99 |
Refinement procedure
| Resolution | 47.810 - 1.400 |
| R-factor | 0.1419 |
| Rwork | 0.140 |
| R-free | 0.16990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.984 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((2.0_5896: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.810 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.056 | 0.438 |
| Rmeas | 0.066 | 0.522 |
| Rpim | 0.035 | 0.280 |
| Total number of observations | 169832 | 5415 |
| Number of reflections | 47847 | 1606 |
| <I/σ(I)> | 8.4 | 2 |
| Completeness [%] | 92.9 | |
| Redundancy | 3.5 | 3.4 |
| CC(1/2) | 0.997 | 0.798 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | Proplex A9 : 0.2M NaCl, 0.1M MES, pH 6.0, 20% PEG2000 MME. TrvaA.01422.c.B2.PB00149 at 20 mg/mL. plate 20301 A9 drop 1, Puck: PSL-0103, Cryo: 20% PEG200 + 80% crystallant |
