9ZHH
Cryo-EM structure of IGFBP7 dodecamer. The 3D refinement was focused on the central part of the dodecamer, which contains 12 IgC2 domains.
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Energy Filter | GIF Bioquantum |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.4 |
| Number of Particle | 2961957 |
| Software | RELION,PHENIX,SerialEM,Gctf,Coot |
| Symmetry | D6 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
