9ZEFExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-05-24 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9199 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.892, 74.610, 133.629 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.390 - 1.550 |
| R-factor | 0.1775 |
| Rwork | 0.177 |
| R-free | 0.19300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.095 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.810 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.073 | 1.071 |
| Rmeas | 0.077 | 1.144 |
| Rpim | 0.026 | 0.399 |
| Number of reflections | 102996 | 5114 |
| <I/σ(I)> | 12.3 | 1.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.6 | 8.2 |
| CC(1/2) | 0.999 | 0.767 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291.15 | 0.1 M NaCl, 18% PEG-3350 |
