9ZEE

QatB-QatC complex in qatABCD anti-phage defense with ATP

Replaces:  9Y02

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-07-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	70.704, 74.366, 134.304
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	67.150 - 1.810
R-factor	0.1824
Rwork	0.181
R-free	0.20350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.769
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.150	1.840
High resolution limit [Å]	1.810	1.810
Rmerge	0.151	1.275
Rmeas	0.157	1.331
Rpim	0.044	0.379
Number of reflections	64929	3179
<I/σ(I)>	9	1.7
Completeness [%]	99.8	99.5
Redundancy	12.3	12.1
CC(1/2)	0.998	0.778

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	291.15	0.1 M MES pH 6.3, 14% PEG-20000, 1 mM MgCl2, 1 mM ATP, 1 mM CDG