9ZDP
Crystal structure of rubredoxin from psychrophilic bacterium Polaromonas glacialis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 73.897, 73.897, 199.351 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 69.290 - 1.830 |
| R-factor | 0.17226 |
| Rwork | 0.171 |
| R-free | 0.20100 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.704 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | HKL2Map |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.290 | 2.042 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.277 | 6.053 |
| Rpim | 0.054 | 0.862 |
| Number of reflections | 36053 | 1803 |
| <I/σ(I)> | 14.1 | 1.6 |
| Completeness [%] | 95.3 | 69.9 |
| Redundancy | 54.1 | 47.9 |
| CC(1/2) | 0.998 | 0.608 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | VAPOR DIFFUSION AGAINST 1.5 M AMMONIUM SULFATE, pH 8.5 |
