EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
EM Data Collection
| Microscope | TFS GLACIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.9 |
| Software | cryoSPARC,ISOLDE,PHENIX,Coot,UCSF ChimeraX,Rosetta |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
