9ZBR

1-methyl-pseudouridine L-21 ScaI Tetrahymena Ribozyme - extended conformation

This is a non-PDB format compatible entry.

EM specimen

Vitrification

Yes

EM Data Collection

Microscope	TFS GLACIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	200
Cs (mm)	2.7
Energy Filter	TFS Selectris X
Detector	FEI FALCON IV (4k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	7.5
Number of Particle	599317
Software	cryoSPARC,ISOLDE,PHENIX,Coot,EPU,UCSF ChimeraX,Rosetta
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION