9ZBQ
1-methyl-pseudouridine L-21 ScaI Tetrahymena Ribozyme
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | TFS GLACIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Energy Filter | TFS Selectris X |
| Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 5.3 |
| Number of Particle | 599317 |
| Software | cryoSPARC,ISOLDE,PHENIX,Coot,EPU,UCSF ChimeraX,Rosetta |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
