9ZBE

Revised structure of a complex between the SNARE Nyv1 and the HOPS Vps33-Vps16 subcomplex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.075
Spacegroup name	P 21 21 2
Unit cell lengths	101.030, 258.936, 75.274
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.470 - 3.050
R-factor	0.186
Rwork	0.184
R-free	0.22380
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.008
RMSD bond angle	0.970
Data reduction software	HKL-3000
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.470	3.190
High resolution limit [Å]	3.050	3.050
Rmerge	0.080	0.734
Rmeas	0.087	0.792
Rpim	0.032	0.296
Total number of observations	276083	31385
Number of reflections	38474	4516
<I/σ(I)>	17.8	2.5
Completeness [%]	99.7
Redundancy	7.2	6.9
CC(1/2)	0.999	0.865

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	TRIS-HCl buffer, 0.8M Na Tartrate, 0.5% w/v PEG 5000 monomethyl ether