9Z75
Crystal Structure of Wild-type Mouse Gamma(S)-Crystallin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 277.15 |
| Detector technology | PIXEL |
| Collection date | 2024-04-03 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 74.056, 74.056, 79.437 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.950 - 1.830 |
| R-factor | 0.16793 |
| Rwork | 0.167 |
| R-free | 0.19278 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.744 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.950 | 1.930 |
| High resolution limit [Å] | 1.830 | 1.900 |
| Rmerge | 0.589 | 2.267 |
| Rmeas | 0.678 | |
| Rpim | 0.331 | |
| Number of reflections | 38290 | 1841 |
| <I/σ(I)> | 8.4 | 1.4 |
| Completeness [%] | 98.9 | |
| Redundancy | 2.1 | |
| CC(1/2) | 0.374 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 0.1 M HEPES, 0.5% v/v Jeffamine |
