9Z5Q
HECT domain of NEDD4-2 complex with a targeted nanobody, nb.C11
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF Bioquantum |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.06 |
| Number of Particle | 1631714 |
| Software | Topaz,PHENIX,cryoSPARC,Leginon,UCSF ChimeraX,Coot |
| Symmetry | C1 |
| CTF correction | NONE |
