9Z2M
Ca2+-bound MthK WT in lipid nanodiscs composed of 14:1PC (75%) and POPG (25%)
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.4 |
| Number of Particle | 837194 |
| Software | RELION,cryoSPARC,PHENIX |
| CTF correction | PHASE FLIPPING ONLY |
