9Z2L

The ubiquitin-associated domain of human thirty-eight negative kinase-1 rigidly fused to the 1TEL crystallization chaperone

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-11-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	38.004, 77.854, 118.995
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.500 - 2.020
Rwork	0.231
R-free	0.27390
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.404
Data reduction software	autoPROC (1.0.5)
Data scaling software	XDS
Phasing software	PHASER (1.20.1_4487)
Refinement software	PDB-REDO

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.500	2.092
High resolution limit [Å]	2.020	2.020
Rmerge	0.092	1.451
Rmeas	0.096	1.647
Rpim	0.028	0.762
Number of reflections	18612	3382
<I/σ(I)>	12.98	0.32
Completeness [%]	75.0	10.95
Redundancy	11.1	4.7
CC(1/2)	0.999	0.456

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	298	0.6 M Magnesium Sulfate, 0.1 Sodium Acetate