9YZ5

human FicD bound with farnesyl pyrophosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-10-18
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	71.021, 76.983, 91.325
Unit cell angles	90.00, 107.20, 90.00

Refinement procedure

Resolution	48.940 - 2.580
R-factor	0.2374
Rwork	0.234
R-free	0.29040
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.120
Data reduction software	XDS
Data scaling software	DIALS
Phasing software	PHENIX
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.940	2.690
High resolution limit [Å]	2.580	2.580
Rmerge	0.274
Number of reflections	29695	202012
<I/σ(I)>	7.3
Completeness [%]	99.6
Redundancy	6.8
CC(1/2)		0.299

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	100 mM Bis-Tris propane (pH 7.5), 200 mM potassium sodium tartrate, and 21% PEG 3350