9YY9

Macrophage Migration Inhibitory Factor 1 from Heligmosomoides polygyrus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97934
Spacegroup name	H 3
Unit cell lengths	71.654, 71.654, 73.125
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.510 - 1.620
Rwork	0.142
R-free	0.17470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.819
Data reduction software	FAST_DP (1.6.2)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.510	1.660
High resolution limit [Å]	1.620	1.620
Rmerge	0.224	0.519
Number of reflections	17545	908
<I/σ(I)>	9	2.4
Completeness [%]	97.5	67.8
Redundancy	4.7	3.9
CC(1/2)	0.965	0.822

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298.15	0.1 M Na Acet 4.5 pH (Buffer), 25 %w/v PEG 3350 (Precipitant)