9YY4

Importin a2 in complex with Lone Star virus non-structural S (NSs) NLS peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-07-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953725
Spacegroup name	P 21 21 21
Unit cell lengths	78.898, 90.144, 100.309
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.680 - 2.350
R-factor	0.1876
Rwork	0.185
R-free	0.22940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.908
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.680	2.440
High resolution limit [Å]	2.350	2.350
Rmerge	0.085	0.704
Number of reflections	30426	3163
<I/σ(I)>	9.3
Completeness [%]	97.5
Redundancy	26.3
CC(1/2)	0.995	0.745

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	296	0.75 M sodium citrate, 0.01 M DTT, 0.1 M HEPES, pH 7.0