9YXW

Crystal structure of HCoV-HKU1 RBD bound by H501-008 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-10-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97
Spacegroup name	C 1 2 1
Unit cell lengths	104.460, 71.760, 174.480
Unit cell angles	90.00, 94.58, 90.00

Refinement procedure

Resolution	57.970 - 2.600
R-factor	0.2522
Rwork	0.252
R-free	0.26670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.441
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.090	2.710
High resolution limit [Å]	2.600	2.600
Number of reflections	39783	2811
<I/σ(I)>	4.8	2.2
Completeness [%]	99.8
Redundancy	3.1
CC(1/2)	0.919	0.703

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Tris pH 8.5, 0.2 M MgCl2, 25% PEG 3350