9YV5

Crystal structure of cytochrome P450 enzyme Bmp7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-09-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000
Spacegroup name	P 32 2 1
Unit cell lengths	101.270, 101.270, 159.692
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.270 - 2.280
R-factor	0.2018
Rwork	0.200
R-free	0.24850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.840
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.320
High resolution limit [Å]	2.280	6.190	2.280
Rmerge	0.148	0.045	0.840
Rmeas	0.169	0.051	0.971
Rpim	0.081	0.024	0.470
Total number of observations	175267
Number of reflections	43400	2334	1984
<I/σ(I)>	5.8
Completeness [%]	98.9	97.5	93.2
Redundancy	4	4	3.4
CC(1/2)	0.987	0.997	0.731

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		278	20% PEG 3350 and 0.2 M of sodium citrate tribasic dihydrate