9YSM

Human DCTPP1 bound to MPD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97933
Spacegroup name	P 1 2 1
Unit cell lengths	61.907, 41.765, 94.208
Unit cell angles	90.00, 93.89, 90.00

Refinement procedure

Resolution	28.770 - 1.810
R-factor	0.1844
Rwork	0.182
R-free	0.22320
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.414
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.770	1.880
High resolution limit [Å]	1.810	1.810
Number of reflections	41611	2846
<I/σ(I)>	11.53
Completeness [%]	94.7
Redundancy	3.1
CC(1/2)	0.997	0.555

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	42% MPD, 80mM NaCl, 80mM MgCl2