9YSF

Crystal structure of EEPD1 EEP domain dimer with Mg(II)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979460
Spacegroup name	C 1 2 1
Unit cell lengths	87.318, 48.210, 73.695
Unit cell angles	90.00, 110.02, 90.00

Refinement procedure

Resolution	38.720 - 1.590
R-factor	0.1677
Rwork	0.166
R-free	0.20430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.001
Data reduction software	XDS (20210323)
Data scaling software	Aimless (0.7.7)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.720	1.630
High resolution limit [Å]	1.590	1.590
Rmerge	0.048	1.274
Rmeas	0.052	1.429
Rpim	0.020	0.634
Number of reflections	37207	2119
<I/σ(I)>	14.21
Completeness [%]	95.3	70.58
Redundancy	6.7	4.9
CC(1/2)	0.999	0.680

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	288	0.1 M Tris, pH 8.5, 0.3 M magnesium chloride, 25% w/v PEG3350