9YRS
E. Coli Glucokinase - K214Q
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | RIGAKU PhotonJet-S |
| Temperature [K] | 297 |
| Detector technology | PIXEL |
| Collection date | 2024-09-13 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 84.971, 84.971, 183.313 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.550 - 2.700 |
| R-factor | 0.143 |
| Rwork | 0.140 |
| R-free | 0.18970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.645 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.550 | 30.550 | 2.830 |
| High resolution limit [Å] | 2.700 | 8.950 | 2.700 |
| Rmerge | 0.146 | 0.053 | 0.682 |
| Rmeas | 0.156 | 0.060 | 0.722 |
| Rpim | 0.052 | 0.025 | 0.234 |
| Number of reflections | 21791 | 667 | 2874 |
| <I/σ(I)> | 11.6 | 24.5 | 3.5 |
| Completeness [%] | 99.9 | 97.3 | 100 |
| Redundancy | 8.8 | 5.1 | 9.4 |
| CC(1/2) | 0.994 | 0.992 | 0.891 |
| Anomalous redundancy | 4.5 | 3.0 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 297 | 100 mM MES / Sodium hydroxide pH 5.5, 100 mM Ammonium citrate dibasic, 20% (w/v) PEG 3350, 5% (v/v) 2-Propanol |
