Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX3 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-07-18 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.953720 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 214.250, 73.510, 59.250 |
| Unit cell angles | 90.00, 94.11, 90.00 |
Refinement procedure
| Resolution | 29.660 - 2.090 |
| R-factor | 0.1837 |
| Rwork | 0.182 |
| R-free | 0.22250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.886 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.660 | 2.140 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.114 | 0.754 |
| Rmeas | 0.843 | |
| Rpim | 0.371 | |
| Number of reflections | 53856 | 3903 |
| <I/σ(I)> | 10.74 | 1.9 |
| Completeness [%] | 98.6 | 96.9 |
| Redundancy | 5.03 | 5 |
| CC(1/2) | 0.998 | 0.667 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.2 M Sodium fluoride, 20% PEG3350 |
